| CTLA-4基因多态性与炎症性肠病的相关性研究 |
| |
| 引用本文： | 蒋益,高劲松,夏冰.CTLA-4基因多态性与炎症性肠病的相关性研究[J].长沙医学院学报,2008(1). |
| |
| 作者姓名： | 蒋益 高劲松 夏冰 |
| |
| 作者单位： | 武汉大学中南医院内科;长沙医学院基础医学系; |
| |
| 基金项目： | 国家自然科学基金资助项目(项目编号:30070350) |
| |
| 摘 要： | 目的炎症性肠病(IBD)的发病机制与T细胞免疫应答过度有关。细胞毒T淋巴细胞相关抗原-4(CTLA-4)主要在已激活的T细胞上表达,通过与CD28竞争与B7结合,抑制T细胞激活,维持免疫系统内环境稳定。CTLA-4基因多态性与一些自身免疫性疾病相关,但未见与炎症性肠病的研究。本文研究CTLA-4基因多态性与炎症性肠病的相关性,旨在阐明炎症性肠病的遗传易感性。方法对68例无血缘关系的湖北汉族炎症性肠病患者(54例溃疡性结肠炎,14例克罗恩病)以及140例正常对照者,采用序列特异性引物PCR方法检测CTLA-4外显子4的3'非转录区包含AT重复序列的特异性等位基因。扩增产物用12%非变性聚丙烯酰胺凝胶电泳,硝酸银染色,部分样品经测序以确定片段长度。结果共发现CTLA-4基因有18种等位基因,其中122bp等位基因与正常对照组比较,在溃疡性结肠炎患者中显著增高(7.4%vs0.3%,P=0.0002/Pc=Sig,OR=22.320,95%CI:2.755～180.80)。结论CTLA-4基因微卫星多态性与溃疡性结肠炎显著相关。
|
| 关 键 词： | 细胞毒T淋巴细胞相关抗原-4(CTLA-4) 基因多态性 炎症性肠病 溃疡性结肠炎 克罗恩病 |
Association between the CTLA-4 gene microsatellite polymorphism and Chinese patients with inflammatory bowel diseases |
| |
| Jiang Yi,Gao jinsong,Xia Bing..Association between the CTLA-4 gene microsatellite polymorphism and Chinese patients with inflammatory bowel diseases[J].Journal of Changsha Medical College,2008(1). |
| |
| Authors: | Jiang Yi Gao jinsong Xia Bing |
| |
| Institution: | Jiang Yi1,Gao jinsong2,Xia Bing1.1. Department of Internal Medicine,Wuhan University Zhongnan Hospital,Wuhan 430071,PR China2.Changsha Medical College,Changsha 410219,PR China |
| |
| Abstract: | Objective Inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation as a result of an exaggerated T -cell response. Cytotoxic T lymphocyte antigen-4 (CTLA-4) expressed mainly on activated T cells inhibits T cell activation by combining B7 through competing CD28 and maintains immune system homeostasis. Polymorphisms in the CTLA-4 gene are known to be associated with several autoimmune diseases, but no studies related to IBD. The aim of the study is to investigate an association bet... |
| |
| Keywords: | Cytotoxic T lymphocyte antigen-4 (CTLA-4) gene polymorphism inflammatory bowel disease ulcerative colitis Crohn\'s disease |
| 本文献已被 CNKI 等数据库收录！ |
|
