| 聚合酶链反应结合生物素探针杂交检测HBV DNA的应用 |
| |
| 引用本文： | 毛申兰,陈常庆,姚光弼,李金兴,杨建华.聚合酶链反应结合生物素探针杂交检测HBV DNA的应用[J].上海医学,1993(3). |
| |
| 作者姓名： | 毛申兰 陈常庆 姚光弼 李金兴 杨建华 |
| |
| 作者单位： | 中科院上海生物工程研究中心，中科院上海生物工程研究中心，上海静安区中心医院，上海静安区中心医院，上海静安区中心医院 |
| |
| 摘 要： | 本研究将聚合酶链反应和生物素探针点杂交相结合,可直接从5μl 血清中检测到10fgHBV DNA。应用此方法检测20例 HBsAg 和 HBeAg 阳性病例,HBVDNA 均阳性;20例 HBsAg 阳性、HBeAg 阴性病例中,有8例阳性;20例健康献血员中,亦有1例检出 HBV DNA。结果表明这是一种灵敏可靠,适合于基础及临床广泛使用的 HBV DNA 非同位素检测法。
|
| 关 键 词： | 乙型肝炎病毒 聚合酶链反应 |
THE COMBINATION OF POLYMERASE CHAIN REACTION AND DNA HYBRIDIZATION ASSAY FOR DETECTION OF SERUM HEPATITIS B VIRUS DNA |
| |
| Mao Shenlan,et al Shanghai Bioengineering Center,Academy Sintca,Shanghai.THE COMBINATION OF POLYMERASE CHAIN REACTION AND DNA HYBRIDIZATION ASSAY FOR DETECTION OF SERUM HEPATITIS B VIRUS DNA[J].Shanghai Medical Journal,1993(3). |
| |
| Authors: | Mao Shenlan Shanghai Bioengineering Center Academy Sintca Shanghai |
| |
| Institution: | Mao Shenlan,et al Shanghai Bioengineering Center,Academy Sintca,Shanghai 200233 |
| |
| Abstract: | A polyrnerasc chain reaction combined with dot hybridization assay for the detection of hepatitis B virus DNA was developed.Serum HBV DNA was amplied using oligonuc- leotide primers specific for S gene.HBV DVA was determined and confirmed by gel elect- rophoresis and dot hybridization with biotin-labelled HBV probe.HBV DNA could be de- tected in the serum using aliquots as 5 microliters,and the detection limit for cloned he- patitis B DNA was 10 fg.Serum specimens of 20 HBsAg-negative healthy donors and 40 HBsAg-positive chronic hepatitis B patients were assayed.HBV DNA were positive in 1 of the 20 healthy persons,all of the 20 cases of HBeAg-positive patients and 8 of the 20 cases of HBeAg-negative patients by using this PCR assay.The result indicates that-the PCR is much more sensitive than those of serological and dot hybridization methods. |
| |
| Keywords: | Hepatitis B virus PCR |
| 本文献已被 CNKI 等数据库收录！ |
