羟基喜树碱对结肠癌LoVo细胞凋亡及 bcl-2、p53基因表达的影响

目的 探讨羟基喜树碱治疗结肠癌LoVo细胞的作用机制。方法 应用四唑盐比色试验、琼脂糖凝胶电泳、DNA末端原位标记染色法研究羟基喜树碱对结肠癌LoVo细胞生长及诱导凋亡的作用，采用Western Blot法分析羟基喜树碱处理结肠癌细胞前后bcl-2、p53基因的表达情况。羟基喜树碱对结肠癌LoVo细胞生长具有较强的抑制作用，可诱导结肠癌细胞凋亡，且其凋亡一浓度及时间依赖性。羟基喜树碱处理结肠癌LoVo细胞后，bcl-2基因表达下降，p53基因表达上升。结论 羟基喜树碱可诱导结肠癌细胞凋亡，可能与下凋bcl-2基因及升高p53基因表达有关。

Effects of hydroxycamptothecin on expressions of bcl-2, p53 gene and apoptosis of colon cancer LoVo cell line

Objective To identify the action mechanism of hydroxycamptothecin on colon cancer cells.Methods Induction of apoptosis of colon cancer LoVo cells were studied by agarose gel electrophoresis assay, TUNEL with hydroxycamptothecin. Results Hydroxycamptothecin possessed a remarkable suppression effect on colon cancer LoVo cell line. Anticancer effect of hydroxycamptothecin would be correlative with the suppression of the induced apoptosis. The effect of induction of apoptosis would be relevant to the concentration and time dependence of hydroxycamptothecin. After colon cancer LoVo cells were treated with hydroxycamptothecin, the gene expression of bcl 2 decreased, but that of p53 was elevated. Conclusion Hydroxycamptothecin can inhibit human colon cancer LoVo cells. The induction of apoptosis can be a very important mechanism in the treatment of colon cancer.