| IRE-BP 1基因在三种淡色库蚊体内表达量的比较分析 |
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| 引用本文： | 谭文彬,王霄,袁常秀,张凤霞,刘建刚,路海,亚白柳,刘永春,李士根,郭永和.IRE-BP 1基因在三种淡色库蚊体内表达量的比较分析[J].中国热带医学,2012,12(4):421-422,426. |
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| 作者姓名： | 谭文彬 王霄 袁常秀 张凤霞 刘建刚 路海 亚白柳 刘永春 李士根 郭永和 |
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| 作者单位： | 1. 济宁医学院病原生物学教研室 山东济宁272033
2. 济宁市人民医院 山东济宁272011 |
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| 基金项目： | 山东省教育厅课题，济宁市科委课题，济宁市优秀中青年科研创新资金计划项目，山东省自然科学基金 |
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| 摘 要： | 目的进行不同品系淡色库蚊铁离子应答元件结合蛋白1基因表达量的分析。方法据二维电泳获得肽段序列设计引物扩增基因片段,荧光定量PCR检测在淡色库蚊抗性品系、敏感品系、现场采集品系蚊体内的表达水平差异。结果成功扩增淡色库蚊铁离子应答元件结合蛋白1基因片段,荧光定量PCR表明该基因在抗性品系蚊体内的表达量是敏感品系的8.22倍,现场品系是敏感品系的5.17倍。结论淡色库蚊铁离子应答元件结合蛋白1基因可做为新的抗性检测及治理基因靶标。
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| 关 键 词： | 淡色库蚊 铁离子应答元件结合蛋白1 荧光定量PCR |
Comparison of expression level of IRE-BP 1 gene in 3 Culex pipiens pallens strains |
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| Institution: | TAN Wen-bin,WANG Xiao,YUAN Chang-xiu,et al.(1.Jining Medical University,Jining 272067,Shandong,P.R.China) |
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| Abstract: | Objective To compare the expression level of IRE-BP 1 gene in different Culex pipiens pallens stains.Methods Degenerate primers were designed on the basis of two-dimensional gel electrophoresis peptide fragment sequence,fluorescence quantitative PCR detect the expression level difference between resistant strain,sensitive strain and local strain of Culex pipiens pallens.Results The IRE-BP 1 gene of Culex pipiens pallens was cloned successfully.FQ-PCR proved the IRE-BP 1 gene expression level of resistant strain is 10.51 times higher than that of sensitive strain,while the expression level of local strain is 5.22 times higher than that of sensitive strain.Conclusion Culex pipiens pallens IRE-BP 1 gene can be used as a new gene target for resistance detection and treatment. |
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| Keywords: | Culex pipiens pallens IRE-BP 1 fluorescence quantitative PCR |
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