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| 儿童天疱疮抗体IgG亚型及与天疱疮抗原的反应性 | |
| 引用本文： | 张国学,王俊秀,梁骏森,吴志华.儿童天疱疮抗体IgG亚型及与天疱疮抗原的反应性[J].中国热带医学,2011,11(8):927-929. |
| 作者姓名： | 张国学 王俊秀 梁骏森 吴志华 |
| 作者单位： | 1. 广东医学院附属医院,广东,湛江,524001 2. 中山市陈星海医院,广东,中山,528415 3. 湛江市第二人民医院,广东,湛江524001 |
| 基金项目： | 湛江市科技计划项目(No.2007-2009) |
| 摘 要： | 目的观察寻常型天疱疮（Pemphigus vulgaris,PV）患儿和一级亲属血清中PV自身抗体（PV-IgG）亚型及其与桥粒芯糖蛋白（Desmoglein,Dsg）反应性,探讨PV-IgG检测的临床意义和PV的发病机制。方法用间接免疫荧光法（Indirect immunofluorescenceI,IF）检测5例PV患儿、12例一级亲属和15例正常对照者血清PV-IgG,并用免疫印迹法（Western blot,WB）检测PV-IgG亚型及其与Dsg1、Dsg3反应性。结果 PV患儿的PV-IgG阳性率和平均抗体滴度明显高于其一级亲属者（P〈0.01）,活动期患儿的PV-IgG阳性率和平均抗体滴度也明显高于缓解期（P〈0.01）;PV患儿中PV-IgG阳性率（80.0%）明显高于一级亲属（25.0%）和正常对照组（0.0%）（P〈0.01）,PV患儿中Dsg3反应性IgG4水平明显高于一级亲属（P〈0.01）,且其在活动期病例中水平也明显高于缓解期（P〈0.05）。结论寻常型天疱疮的发生与PV-IgG水平有关;Dsg3是PV的主要自身抗原I;gG4是PV的主要致病抗体。 |
| 关 键 词： | 儿童寻常型天疱疮 桥粒芯糖蛋白I gG 间接免疫荧光法 免疫印迹法 |
Investigation of pemphigus vulgaris-IgG subclasses and their reactivity with desmoglein in pemphigus children |
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| ZHANG Guo-xue,WANG Jun-xiu,LIANG Jun-sen,et al..Investigation of pemphigus vulgaris-IgG subclasses and their reactivity with desmoglein in pemphigus children[J].China Tropical Medicine,2011,11(8):927-929. | |
| Authors: | ZHANG Guo-xue WANG Jun-xiu LIANG Jun-sen |
| Institution: | ZHANG Guo-xue,WANG Jun-xiu,LIANG Jun-sen,et al.(1.The Affiliated Hospital,Guangdong Medical College,Zhanjiang,524001,P.R.China) |
| Abstract: | Objective To observe the pemphigus vulgaris autoantibody(PV-IgG) subclasses and their reactivity with desmoglein(Dsg) in PV children and their first-degree relatives,and to investigate the clinical significance of PV-Ab detection and the pathogenesis of PV.Methods Serum PV-Ab levels,and PV-IgG subclasses and their reactivity with Dsg1 and Dsg3 in 5 PV children,12 first-degree relatives and 15 healthy individuals were determined by indirect immunofluorescence(IIF) and Western blot(WB),respectively.Results Th... |
| Keywords: | Pemphigus vulgaris children Desmoglein IgG Indirect immunofluorescence Western blot |
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