| 弓形虫MIC8基因的克隆及真核表达质粒的构建 |
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| 引用本文： | 赵焕阁,李煜,谭光宏,黄凤迎,王华,郭峻莉,周松林,黄用豪.弓形虫MIC8基因的克隆及真核表达质粒的构建[J].中国热带医学,2011,11(7):805-807. |
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| 作者姓名： | 赵焕阁 李煜 谭光宏 黄凤迎 王华 郭峻莉 周松林 黄用豪 |
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| 作者单位： | 1. 海南医学院海南省热带病重点实验室,海南海口,571101
2. 解放军第187医院,海南海口,571159 |
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| 基金项目： | 海南省自然科学基金“弓形虫MIC8真核表达载体构建及免疫原性研究”(No.809018)资助 |
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| 摘 要： | 目的构建弓形虫微线体MIC8的真核表达重组质粒。方法设计合成弓形虫MIC8引物，运用PCR方法扩增其基因片段克隆至真核表达质粒pCDNA3．1，从而构建重组表达质粒pCDNA3．1-MIC8。结果PCR扩增弓形虫MIC8基因序列正确，构建的重组表达质粒pCDNA3．1-MIC8经PCR、HindⅢ和XbaⅠ双酶切和测序鉴定正确。结论成功获得真核表达重组质粒3-pCDNA3．1－MIC8，为进一步研究弓形虫疫苗的免疫保护性奠定基础。
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| 关 键 词： | 弓形虫 MIC8 真核表达质粒 |
Construction of eukaryotic recombinant expression plasmid containing Toxoplasma gondii MIC8 |
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| ZHAO HUan-ge,LI Yu,TAN Guang-hong,et al..Construction of eukaryotic recombinant expression plasmid containing Toxoplasma gondii MIC8[J].China Tropical Medicine,2011,11(7):805-807. |
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| Authors: | ZHAO HUan-ge LI Yu TAN Guang-hong |
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| Institution: | ZHAO HUan-ge,LI Yu,TAN Guang-hong,et al.(1.Key Laboratory for Tropical Diseases,Hainan Medical College,Haikou 571101,Hainan,P.R.China) |
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| Abstract: | Aim To construct an eukaryotic expression recombinant plasmid containing microneme 8(MIC8) of Toxoplasma.gondii.Methods MIC8 gene fragment was amplified by PCR with primers and cloned into pCDNA3.1 to construct eukaryotic expression plasmids pCDNA3.1-MIC8.ResuLts The gene fragmentent encoding MIC8 was amplified correctly by PCR.The recombinant eukaryotic expression plasmid pCDNA3.1-MIC8 was accredited and confirmed to be right by sequencing.ConcLusion The successful construction of recombinant eukaryotic ex... |
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| Keywords: | Toxoplasma gondii Microneme MIC8 Eukaryotic expression plasmid |
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