肺炎支原体感染的血清学检测方法比较

目的比较几种检测方法对呼吸道感染患者血清肺炎支原体（MP）特异性抗体IgM检测的检出率。方法以冷凝集试验（CAT）、明胶颗粒凝集法（PLA）、酶联免疫吸附试验（ELISA）3种方法对95例呼吸道感染患者血清肺炎支原体IgM抗体进行检测。结果冷凝集试验、明胶颗粒凝集法、酶联免疫吸附试验检测血清肺炎支原体IgM抗体的敏感性分别为69.1%、96.4%和90.9%,特异性分别为75%、100%和90%,准确度分别为68.4%、97.9%和90.5%。用配对资料四格表法对三种方法的检出率进行分析,经SPSS软件统计分析,冷凝集试验与明胶颗粒凝集法和酶联免疫吸附试验相比较,差异有统计学意义（P〈0.05）,酶联免疫吸附试验与明胶颗粒凝集法相比较,差异无统计学意义（P〉0.05）。结论冷凝集试验与另外两种方法相比有显著差异,而酶联免疫吸附试验与明胶颗粒凝集法两种方法没有显著差异,两者联合应用可以提高阳性率,对临床治疗起到及时的指导。

Comparison of serologic detections of Mycoplasma pneumonia infection

Aim To evaluate the detection rate,sensitivity,specificity of serologic detection of IgM antibody to Mycoplasma pneumonia in patietns with respiratory tract infection.Methods Cold agglutination test（CAT）,polystyrene latex agglutination reaction（PLA）,enzyme linked immunosorbent assay（ELISA）were used in the detection of IgM antibody to Mycoplasma pneumonia in 95 patients with respiratory tract infection.Results The sensitivity of CAT,PLA and ELISA were 69.1%,96.4% and 90.9%;the specificity were 75%,100% and 90%;the accuracy were 68.4%、97.9% and 90.5%.Cold agglutination test compared with polystyrene latex agglutination reaction（PLA）,P0.05（P=0.001）;The results of CAT in comparison with ELISA）showed significant difference（P0.05）（P=0.008）;the results of ELISA）in comparison with LLA also showed significant difference（P0.05）（P=0.581）.Conclusion The positive rate in detection of IgM antibody to Mycoplasma pneumonia by PLA in combination with ELISA can be enhanced.