568份疑似甲型H1N1流感病例标本病原学检测结果分析

目的对河池市568份疑似甲型H1N1流感病例标本进行核酸检测,为甲型H1N1流感疫情的防控提供依据。方法对疑似甲型H1N1流感病例标本用实时荧光定量RT-PCR法（Real-time RT-PCR）检测,检测项目为A型流感病毒核酸、B型流感病毒核酸和甲型H1N1流感病毒核酸,对A型流感病毒核酸阳性而B型流感病毒核酸和甲型H1N1流感病毒核酸均阴性标本再进行季节性流感病毒H1N1、H3N2亚型流感病毒核酸检测。结果检测疑似病例标本568份,其中甲型H1N1流感病毒核酸阳性334份,季节性流感病毒H1N1亚型核酸阳性14份,季节性流感病毒H3N2亚型核酸阳性25份,A未分型流感病毒核酸阳性40份。结论实验室检测为甲型H1N1流感疫情的确认、防控以及病人的处理提供了依据,对提高实验室检测技术的敏感性、特异性和实效性具有重要的意义。

Results of detection of 568 suspected influenza A virus subtype H1N1 patients

Aim To detect influenza A virus subtype H1N1 in Hechi City from suspected patients by nucleic acid assay.Methods The nucleic acids of influenza A and B viruses and nfluenza A virus subtype H1N1 were simultaneously detected from 568 suspected influenza A virus subtype H1N1 patients by using real time quantitative RT-PCR method（Real-time RT-PCR）.Seasonal influenza virus subtype H1N1 and H3N2 would detected from the samples positive for influenza A virus nucleic acid,while negative for influenza B virus and influenza A virus subtype H1N1 nucleic acid.Results In the 568 suspected samples,there were 334 samples positive for influenza A H1N1 influenza virus nucleic acid and 14 positive for seasonal influenza virus subtype H1N1 nucleic acid positive,25 positive for seasonal influenza virus subtype H3N2 nucleic acid positive and 40 positive for no-differentiated influenza A virus nucleic acid.Conclusion Laboratory tests provide a basis for sensitive,specific and efficient confirmation and control of influenza A（H1N1）.