甘泰康对小鼠四氯化碳肝损伤的影响及其机理探讨

目的 观察甘泰康对四氯化碳(CCl4)肝损伤小鼠的影响,并探讨其作用机理。方法 参照《保健食品功能学评价程序和方法》中护肝试验方法,以不同剂量的甘泰康给小鼠灌胃30天后,第31天模型对照组和三个剂量组小鼠腹腔注射0.1％CCl4植物油溶液0.1ml/10g·bw,禁食20h后采血,测血清ALT、AST、GST、TP、ALB、CHOL、TG、GLU、CHE、MDA及肝组织MDA、GST含量,光镜下观察肝组织的病理变化。结果 甘泰康可显著降低由CCl4所致的 ALT、AST、GST、TG升高,可明显对抗由CCl4引起的血清ALB、GLU下降,能明显改善CCl4所致的肝组织病理学损伤,降低肝内MDA含量,对血清TP、CHOL、CHE、MDA及肝组织GST含量影响不明显。结论甘泰康对小鼠CCl4肝损伤有一定的防护作用,其作用机理可能与降低肝内脂质过氧化反应、改善机体免疫调节功能、保护肝细胞膜、增强机体的解毒、排毒功能有关。

Effects and Mechanisms of Gantaikang on Liver Injury Induced by Carbon Tetrachloride in Mice

Objective To observe the effects of Gantaikang on liver injury induced by carbon tetrachloride in mice and investigate the mechanisms.Methods According to liver - injury - prevention test methods in Health Food Functional Assessment Procedure and Test Methods, the mice was given orally with different doses of Gantaikang for 30 days. On 31st day, the mice in model control group and three dosed groups were administered intraperitoneally with 0.1% carbon tetrachloride (CCl4) in vegetable oil. After fasting for 20 hours, blood was collected, and serum levels of ALT, AST, GST,TP, ALB,CHOL,TG,GLU,CHE,MDA and MDA,GST contents in liver were detected. Pathological changes in livers were observed by light - microscopy. Results Gantaikang could significantly ameliorate the increased serum levels of ALT,AST,GST and TG induce by CCU .markedly prevent CCU - induced decrease of serum ALB and GLU,evidently improve CCl4 - induced liver histopathological injuries, and reduced elevated MDA content in liver, but had no marked effects on serum TP,CHOL,CHE,MDA and GST activity in liver. Conclusion Gantaikang had prevention effects against CCl4- induced liver injury in mice.The mechanisms may be related with its effects of reducing lipid peroxidation product,improving body's immunoregulatory function,stabilizing hepatocelluar membrane and enhancing the detoxification ability of the body.