臭氧暴露对COPD大鼠气道炎症及肺组织NF-κB表达的影响

目的研究环境因素中臭氧与慢性阻塞性肺病发病率升高之间的关系；探讨臭氧暴露对COPD个体气道炎症及肺组织NF-κBP65蛋白表达的影响。方法雄性sD大鼠30只，随机分为三组，对照组，COPD模型组，模型叠加臭氧暴露组，每组10只。每日熏吸香烟和两次气管内滴人200ug脂多糖建立大鼠COPD模型，模型加臭氧暴露组在此基础上进行1次急性臭氧暴露，暴露时间为4h。观察记录各组大鼠一般情况，HE染色检测大鼠肺组织病理改变，支气管肺泡灌洗液行瑞士染色，显微镜下进行炎症细胞分类计数，免疫组化检测肺组织NF-κBP65蛋白表达量。结果模型组大鼠的肺组织损伤及病理变化总分显著高于对照组大鼠（P〈O．01），臭氧组总分显著高于模型组（P〈O．01）；模型组大鼠BAⅡ（支气管肺泡灌洗液）中炎性细胞总数高于对照组，臭氧组高于模型组；模型组大鼠肺组织NF-κB P65蛋白表达量显著高于对照组（P〈0．01），臭氧组则显著高于模型组（P〈0．05）。结论臭氧暴露可显著加重COPD气道炎症及NF-κB在肺组织中的表达。

Effect of ozone exposure on airway inflammation and NF-κB expression in lung tissue of COPD rats.

Objective To explore the effect of ozone exposure on airway inflammation and NF -κB （nuclear factor-κ B）P65 protein expression in lung tissue of COPD rats. Methods Thirty male SD rats were randomly divided into control group,model group and ozone exposure group each consisted of 10 rats.The COPD model was established by intratracheal instillation of lipopolysaecharide （LPS）200ug and exposure to cigarette smoke twice daily.The ozone exposure group on the basis of this add ozone exposure. HE staining detection of pathology change in lung tissue of rats, Counting BALF inflammatory cells under optical microscope, Immunohistochemieal detection of NF-κB P65 protein expression in lung tissue. Results The lung tissue injury and pathological change score in COPD model group rats significantly higher than the nomal group rats（P〈0.01 ）;score of ozone group significantly higher than C OPD model group （P〈0.01）;The total number of inflammatory cells in BALF of COPD model group rats significantly higher than the hemal group. Ozone group was significantly higher than model group; The lung tissue NF-κB P65 protein expression in COPD model group rats was significantly higher than the nomal group （P〈0.01）;The lung tissue NF-κB P65 protein expression in ozone group is significantly higher than COPD model group （P〈0.05）. Conclusions Ozone stimulation can significantly increase the airway inflammation and NF-κB protein expression in lung tissue of COPD.