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慢性乙肝患者淋巴细胞亚群水平与肝纤程度关系的研究
引用本文:李莎莎,;邓志勇,;付茂英,;秦海峰,;何敖林,;王楷文.慢性乙肝患者淋巴细胞亚群水平与肝纤程度关系的研究[J].中国热带医学,2014(8):990-992.
作者姓名:李莎莎  ;邓志勇  ;付茂英  ;秦海峰  ;何敖林  ;王楷文
作者单位:[1]昆山市第一人民医院中心实验室,江苏昆山215300; [2]昆山市第一人民医院感染性疾病科,江苏昆山215300; [3]昆山市第一人民医院放免科,江苏昆山215300
摘    要:目的观察HBV病毒水平和淋巴细胞亚群变化在肝纤维化进程中的价值。方法采用荧光定量PCR、流式细胞术及放射免疫法(RIA)方法检测100例慢性乙型肝炎患者淋巴细胞亚群和肝纤维化指标,同期选取健康对照组并进行统计学处理。结果与健康对照组相比,DNA阴性组淋巴细胞分型的差异无统计学意义,但肝纤指标则明显增加(PCⅢ:118.43±17.83 vs 79.43±6.08;Ⅳ-C:98.26±10.23vs48.66±6.26;LN:112.48±13.63vs87.04±6.73;HA:98.28±15.68vs33.00±6.66;P均0.05);低病毒载量组与DNA阴性组相比,CD8+T细胞增多(36.18±6.88vs28.13±5.11,P0.05),高病毒载量组与DNA阴性组相比,CD4+T细胞显著减少(33.54±4.99vs38.38±5.81,P0.05)、CD8+T细胞明显增多(38.15±7.92vs28.13±5.11,P0.05)、CD4+/CD8+下降(1.06±0.41vs1.42±0.39,P0.05),但肝纤四项均明显增高(P0.05)。结论慢性HBV感染患者在疾病进展中选择合适的时机联合检测,将为早期干预和抗病毒治疗提供科学依据。

关 键 词:慢性乙型肝炎  淋巴细胞亚群  HBV  DNA载量

Relationship between level of lymphocyte subpopulations and index of liver fibrosis in patients with chronic HBV infection
Institution:LI Sha-sha, DENG Zhi-yong, FU Mao-ying, QIN Hai-feng, HE Ao-lin, WANG Kai-wen.( 1.Center Laboratory, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu, China)
Abstract:Objective To investigate relationship between the changes of peripheral blood lymphocyte subsets and the four indexes of liver fibrosis with chronic hepatitis B virus infection. Methods A total of 100 patients with chronic HBV infection were enrolled and 30 people as health controls. Composition of peripheral lymphocyte subpopulations was determined by flow cytometry. The four indexes of liver fibrosis were detected by RIA. Serum HBV DNA load was assessed by quantitative real-time PCR. Result Compared with the normal controls, there was no significant difference in lymphocyte subsets in DNA negative group, but the liver fibrosis markers increased (PCⅢ: 118.43±17.83 vs 79.43±6.08; Ⅳ-C: 98.26±10.23vs48.66±6.26; LN: 112.48±13.63vs87.04±6.73; HA: 98.28±15.68vs33.00±6.66; all P〈0.05).The percentages of CD8+T in low load group increased as compared with negative group (36.18±6.88vs28.13±5.11;P〈0.05), compared with negative group, the percentages of CD4+T in high load group reduced remarkably(33.54±4.99vs38.38±5.81, P〈0.05)CD8+T in high load group increased obviously(36.18±6.88vs28.13±5.11,P〈0.05)and CD4+/CD8+ratio in high load group decreased(1.06±0.41vs1.42±0.39, P〈0.05), moreover, the liver fibrosis markers were obviously enhanced. Conclusion Combined detection of peripheral blood lymphocyte subsets and liver fibrosis makers in patients with chronic hepatitis B virus infection are of important significance for diagnosis and treatment of the disease.
Keywords:Chronic hepatitis B virus infection  Lymphocyte subpopulations  Hepatitis B virus DNA load
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