慢性乙型肝炎患者外周血IL-7与细胞免疫的关系

目的研究慢性乙型肝炎(CHB)患者外周血IL-7含量与T淋巴细胞亚群、调节性T细胞百分比的关系,探究IL-7对慢性乙型肝炎细胞免疫作用的影响。方法慢性乙型肝炎患者组、慢性乙型肝炎病毒(HBV)携带组、健康对照组,采用ELISA法检测受试者外周血IL-7含量,流式细胞仪技术检测外周血T淋巴细胞亚群和CD4+CD25+调节性T细胞百分比。结果 CHB组、HBV携带组、健康对照组外周血IL-7含量分别为(1.68±0.58)ng/mL、(3.83±0.62)ng/mL和(6.25±0.32)ng/mL,3组间存在显著性差异(P0.05);CHB组、HBV携带组、健康对照组外周血T细胞亚群CD4+T淋巴细胞分别为(31.3±3.3)%、(38.1±6.8)%、(43.2±9.4)%,CD8+T淋巴细胞分别为(24.8±5.2)%、(28.1±3.4)%、(30.5±8.6)%;CD4+CD25+调节性T细胞分别为(10.9±1.3)%、(7.4±1.0)%、(4.2±1.1)%。CHB组外周血IL-7明显低于HBV携带组及健康对照组,且与CD4+T细胞百分比呈负相关(r1=-0.58,P0.05),与CD8+T细胞百分比呈正相关(r2=0.63,P0.05),与CD4+CD25+调节性T细胞百分比呈负相关(r3=-0.43,P0.05)。结论 CHB患者外周血IL-7负调控调节性T细胞,对CD8+T细胞的细胞免疫有促进作用。

Relationship between concentration of IL-7 and cellular immunity in peripheral blood of the patients with chronic hepatitis B

Objective To study the relationship of the concentration of IL-7 with the percentage of T lymphocyte subsets and regulatory T cells in peripheral blood of the patients with chronic hepatitis B and explore the effects of IL-7 on cellular immune function. Methods The 48 patients were divided into CHB group （30 cases） and HBV carrier group （18 cases）. In addition, there was a health control group（14 cases）.The concentration of IL-7 in the peripheral blood in the chronic hepatitis B group , the chronic hepatitis B virus （HBV） carrier group, and the healthy control group were measured by ELISA and the percentage of T lymphocyte subsets and CD4＋CD25＋ regulatory T cells were detected by the flow cytometry. Results The concentration of IL-7 was （1.68 ± 0.58） ng/ml in the CHB group, （3.83 ± 0.62） ng/ml in HBV carriers group and （6.25 ± 0.32） ng/mL in the healthy control group, there were significant differences among the 3 groups （P〈0.05）. The percentage of CD4＋T cells in the CHB group, the HBV carriers group, and the healthy control group were （31.3 ± 3.3） %, （38.1 ± 6.8）% and （43.2 ± 9.4）%, respectively; CD8＋T cells were （24.8 ± 5.2）%, （28.1 ± 3.4）% and （30.5 ± 8.6）% ,respectively; CD4＋CD25＋ regulatory T cells were （10.9 ± 1.3）%, （7.4 ± 1.0）% and （4.2 ± 1.1）% ,respectively. The concentration of IL-7 of the CHB group was significantly lower than that of HBV carrier group or healthy control group, it was negatively correlated with the percentage of CD4＋T cells （r1=-0.58, P〈0.05） and CD4＋CD25＋ regulate T cells （r3=- 0.43, P〈0.05）. However, it is positively correlated with the percentage of CD8＋T cells （r2=0.63, P〈0.05）. Conclusion IL-7 regulates negatively of regulatory T cells, and promotes the CD8＋T cellular immunity of the patients with chronic hepatitis B.