大鼠腹膜间皮细胞中转化生长因子β1对TNF-α表达的影响 |
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引用本文: | 叶玲,祝胜郎,陈结慧,朱恒梅.大鼠腹膜间皮细胞中转化生长因子β1对TNF-α表达的影响[J].中国热带医学,2013,13(6):667-670. |
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作者姓名: | 叶玲 祝胜郎 陈结慧 朱恒梅 |
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作者单位: | 广东医学院附属南山医院肾内科,广东深圳,518052 |
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摘 要: | 目的 探讨在大鼠腹膜间皮细胞(RPMC)中转化生长因子β1 (TGF-β1)对肿瘤坏死因子-α (tumor necrosis factor-α,TNF-α)表达的影响及其机制.方法 在体外培养的大鼠腹膜间皮细胞模型中,加入TGF-β1(10ng/ml)预刺激,研究RPMC中肿瘤坏死因子-α(TNF-α)的表达;体外转染Smad7至RPMC后,研究其对TGF-β1(10 ng/ml)刺激后RPMCs中TNF-α和p38表达的影响.p38抑制剂SB203580(10umol/L)预处理RPMCs后,加入TGF-β1(10 ng/ml)刺激,研究抑制p38信号通路后对TNF-α表达的影响.结果 RT-PCR结果显示,与0h对照组相比,3h、6h、12hTGF-β1刺激组TNF-α表达明显增加(P<0.05).上调表达Smad7抑制TGF-β1刺激RPMC产生TNF-α的作用,p38抑制剂SB203580亦能抑制TGF-β1刺激RPMC表达TNF-α的作用.TGF-β1参与p38磷酸化的活化,Smad7可抑制TGF-β1对它的激活反应;与正常对照组比较,TGF-β1刺激组磷酸化(p)-p38的表达显著增加(P<0.05),与TGF-β1刺激组比较,Smad7治疗组p-p38的表达显著减弱(P<0.05).结论 在大鼠腹膜间皮细胞中,TGF-β1能促进TNF-α的表达,其作用机制可能是依赖活化p38MAPK信号通路来实现的.
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关 键 词: | 转化生长因子-β1 Smad7 p38丝裂原活化蛋白激酶 肿瘤坏死因子-α |
Effect of transforming growth factor-β1 on TNF-α expression in rat peritoneal mesothelial cells |
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Institution: | YE Ling, ZHU Sheng-lang,CHEN Jie-hui,et al..(Department of Nephrology,Affiliated Nanshan Hospitai of Guangdong Medical College,Shenzhen 518052,Guagndong,P.R.China.) |
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Abstract: | Objective To observe the effect of transforming growth factor-β1(TGF-β1)on the expression of tumor necrosis factor-α(TNF-α) in rat peritoneal mesothelial cells(RPMCs) and its machanism.Method TGF-β1-induced RPMCs model in vitro was established,the expression of TNF-α in the TGF-β1-induced RPMCs was explored;the intervention of Smad7 on the expression of TNF-α and p38 signal proteins induced by TGF-β1 in RPMCs were observed,and the intervention of p38 inhibitor SB203580 on the expression of TNF-α induced by TGF-β1 in RPMCs was also observed.Results TGF-β1 could stimulate TNF-α expression in RPMCs;Compared with control group,TNF-α mRNA levels were significantly increased 3h,6h and 12h after treatment with TGF-β1(p0.05)..Over-expressed Smad7 could reduce the expression of TNF-α induced by TGF-β1(p0.01),p38 inhibitor also could reduce the expression of TNF-α induced by TGFβ1(p0.01).TGF-β1 could activate p38 signaling pathway,over-expressed Smad7 could inhibit this role of TGF-β1.Comparing with control group,the expression of p-p38 was increased in TGF-β1-stimulated-group;comparing with TGF-β1 stimulated-group,the expression of p-p38 was reduced in Smad7 gene transfer group.Conclusion TGF-β1 can stimulate the expression of TNF-α in rat peritoneal mesothelial cells,and this role is p38 MAPK dependent. |
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Keywords: | Transforming growth factor beta Smad7 P38mitogen-activated protein kinase tumor necrosis factor-α |
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