| 家蝇乙酰胆碱酯酶基因的克隆与序列分析 |
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| 引用本文： | 方晓东,黄俊生.家蝇乙酰胆碱酯酶基因的克隆与序列分析[J].中国热带医学,2006,6(6):950-952. |
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| 作者姓名： | 方晓东 黄俊生 |
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| 作者单位： | 热带作物生物技术国家重点实验室,海南,海口,571101 |
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| 基金项目： | 本项目得到国家科技部“科技基础性工作和社会公益研究专项”（编号：2004DIB3J073）和科技部“国家科技基础条件平台工作”（编号：2004DKA30560）等资助 |
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| 摘 要： | 目的克隆家蝇Ace基因并进行表达,并对家蝇AChE序列进行分析,为利用基因工程进行酶改造提供必要的参考. 方法结合已有的文献资料,利用生物信息学方法对家蝇AChE的序列进行分析,包括密码子偏爱性、系统发生、三维结构预测等. 结果成功克隆家蝇Ace基因,并利用同源模建方法获得了家蝇AChE的三维结构,以果蝇AChE为参考,判断了家蝇AChE应属于AChE 2家族. 结论根据所得家蝇AChE的三维模型从理论上分析了因AChE中一个氨基酸突变导致家蝇产生有机磷抗性的可能原因,并为半理性改造家蝇乙酰胆碱酯酶提供了三维模型.
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| 关 键 词： | 乙酰胆碱酯酶 序列分析 进化树 三维结构 同源模建 |
| 文章编号： | 1009-9727(2006)06-950-03 |
| 收稿时间： | 03 10 2006 12:00AM |
| 修稿时间： | 2006年3月10日 |
Cloning and sequence analysis of AChE gene from Housefly (Musca domestica) |
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| FANG Xiao-dong,HUANG Juns-sheng.Cloning and sequence analysis of AChE gene from Housefly (Musca domestica)[J].China Tropical Medicine,2006,6(6):950-952. |
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| Authors: | FANG Xiao-dong HUANG Juns-sheng |
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| Institution: | State Key Biotchnology Laboratory for Tropical Crops Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, P. R. China |
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| Abstract: | Objective To clone acetylcholinesterase(AChE) gene from Housefly (Musca domestica) in order to provide necessary reference for genetic engineering study. Methods The sequence of AchE was, by means of bioinformatics, analyzed including codon bias, systemic genesis, predicting of three-dimentional structure by using bioinformatics.Results AChE gene from housefly was cloned and AChE three-dimentional structure was obtained by homologous modeling. AChE,belonged to AChE 2 family, from fruitfly was taken as reference. Conclusion Based on housefly's AChE three-dimentional structure it is theoretically indicating the possibility that a mutation of an amino acid in Housefly's AChE can lead to the resistance of housefly to organic phosphorus pesticide. |
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| Keywords: | Acetylcholinesterase Sequence analysis Three - dimentional structure Homologous modeling |
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