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广西2010年急性出血性结膜炎暴发病原基因型分析
引用本文:陈敏玫,居昱,闭福银,谭毅,何为涛,莫毅,杨进业.广西2010年急性出血性结膜炎暴发病原基因型分析[J].中国热带医学,2011,11(12):1462-1463.
作者姓名:陈敏玫  居昱  闭福银  谭毅  何为涛  莫毅  杨进业
作者单位:广西壮族自治区疾病预防控制中心,广西南宁,530028
摘    要:目的确定引起2010年广西急性出血性结膜炎(Acute Hemorrhagic Conjunctivitis,AHC)流行的病原及分子进化特征。方法流行期间采集门诊AHC就诊病例眼结膜拭子标本20份,用聚合酶链反应(PCR)方法同时检测腺病毒、人肠道病毒70型(Human Enterovirus 70,HEV70)和柯萨奇病毒A组24型变种(Coxsackievirus A24 variant,CoxA24v)核酸,对CoxA24v 3C区和VP1区核苷酸序列进行分析。结果 CoxA24v核酸阳性11份,阳性率为55.0%,HEV70和腺病毒核酸检测结果均为阴性。广西各株间的3C区和VP1区核苷酸序列一致性分别为99.6%~100.0%和98.3%~100.0%,在进化树中聚集在基因3群中,形成独立分支。结论引起2010年广西AHC流行的主要病原为CoxA24v,属于基因3群,在进化树中独成一个新的分支。

关 键 词:急性出血性结膜炎  柯萨奇病毒A组24型变种  分子进化分析

Genotypic analysis of pathogens caused outbreak of acute hemorrhagic conjunctivitis in Guangxi in 2010
CHEN Min-mei,JU yu,BI Fu-yin,et al..Genotypic analysis of pathogens caused outbreak of acute hemorrhagic conjunctivitis in Guangxi in 2010[J].China Tropical Medicine,2011,11(12):1462-1463.
Authors:CHEN Min-mei  JU yu  BI Fu-yin  
Institution:CHEN Min-mei,JU yu,BI Fu-yin,et al.(Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention,Nanning,530028,Guangxi,P.R.China)
Abstract:Objective To identify the pathogens of the AHC outbreak in Guangxi,2010.Methods Twenty eye conjunctival swabs were collected from 20 outpatients,adenovirus,human enterovirus 70(HEV70)and Coxsackievirus A24 variant(CoxA24v)genes were tested by PCR.Results Eleven samples were identified positive for CoxA24v,none was positive for HEV70 and adenovirus.The sequences of 3C and VP1 region from 6 samples were analysed,the identify were 99.6%~100.0% and 98.3%~100.0% on nucleotide level,phylogeny analysis based on 3C...
Keywords:Acute hemorrhagic conjunctivitis  Coxsackievirus A24 variant  Phylogenetic analysis  
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