不同品系小鼠感染华支睾吸虫后IgG及亚类的动态观察

目的观察不同品系小鼠感染华支睾吸虫后，血清中特异性抗体IgG、IsGI／IgG2a的动态变化。方法NaOH消化法粪检虫卵，ELISA法测定不同品系小鼠感染后4、8和12w血清中抗体IsG、IsGI／IgG2a的动态变化。结果三个品系小鼠血清IsG均于感染后第4～8w升高，约在第10w达到高峰，此后开始下降并维持至观察结束的第12w；IgGI／IgG2a比值感染后逐渐增高，并持续至第12w。结论华支睾吸虫感染的免疫应答发生了由Th1免疫应答向Th2免疫应答转变的趋势。

Studies on the dynamic changes of IgG antibodies and subclasses in sera from different strains of mice Infected with Clonorchis sinensis

Objective To observe the dynamic changes of specific IgG, IgG1/IgG2a against the adult antigen of Clonorehis sinensis in sera from different strains of mice after infection. Methods The eggs from feces of the 3 strains of mice were detected with NaOH digestion method and the ELISA were used for the quantitative determination of specific IgG, IgG1/IgG2a against the adult antigen of Clonorehis sinensis in sera 4w, 8w, 12w after infection. Results The specific IgG in 3 strains of mice increased at the 4th - 8th week after infection, which reached the peak at about the 10^th week post infection, then began to decrease;IgG1/IgG2a in 3 strains of mice continuously increased to the 12^th week after infection. Conclusion The immunological reaction in mice with C. sinensis shows a tendency from Thl - type predominant response to Th2 - type predominant response along with the extension of the infection.