| 应用插入序列PCR进行大肠杆菌O157:H7基因分型的研究 |
| |
| 引用本文： | 郭喜玲,史智扬,汪华,曾晓燕,顾玲,李显.应用插入序列PCR进行大肠杆菌O157:H7基因分型的研究[J].中国热带医学,2005,5(7):1444-1446. |
| |
| 作者姓名： | 郭喜玲 史智扬 汪华 曾晓燕 顾玲 李显 |
| |
| 作者单位： | 江苏省疾病预防控制中心江苏省医学重点实验室肠道致病菌实验室,江苏,南京,210009 |
| |
| 摘 要： | 目的对不同地区、不同宿主大肠杆菌O157：H7分离株进行基因分型。方法应用插入序列PCR方法，对反应体系中dNTPs、引物以及镁离子浓度等反应条件进行优化。扩增产物通过琼脂糖凝胶电泳检测和分析。结果14株不同来源O157：H7分离株根据插入序列PCR图谱可分为A、B、C、D四个基因型。不同地区O157：H7菌株存在不同的基因型；同一地区、毒力基因谱相同的O157：H7菌株基因型也不相同；不同宿主O157：H7菌株也存在相同的基因型。结论插入序列PCR技术用于大肠杆菌O157：H7的基因分型，简便、快速、敏感，对大肠杆菌O157：H7的分子流行病学研究具有重要价值。
|
| 关 键 词： | 大肠杆菌 O157：H7基因分型 插入序列 聚合酶链反应 |
| 文章编号： | 1009-9727（2005）07-1444-03 |
| 收稿时间： | 2005-08-03 |
Study on the application of insert sequence PCR in genotyping of E. Coli O157:H7 |
| |
| GUO Xi - ling, SHI Zhi - yang, WANG hua,et al..Study on the application of insert sequence PCR in genotyping of E. Coli O157:H7[J].China Tropical Medicine,2005,5(7):1444-1446. |
| |
| Authors: | GUO Xi - ling SHI Zhi - yang WANG hua |
| |
| Institution: | Zhejiang Provincial Center for Disease Control and Prevention, Nanjing 210009, Zhejiang, P. R. China |
| |
| Abstract: | Objectiwe To perform genetic categorization of Ecoli O_ 157 :H_7 strains acquired from hosts in different districts. Methods IS-PCR was performed and PCR conditions were optimized. Amplification products were analyzed by agarose gel electrophoresis. Results A total of 14 strains of O_ 157 :H_7 were further categorized as genetic groups A, B, C and D. Both strains from different regions and strains with similar virulence gene profiles in the same region could be further differentiated by IS-PCR, while strains isolated from different individuals may share the same profile. Conclusions IS-PCR is a fast sensitive method for Ecoli O_ 157 :H_7 genetic categorization. It may possess significance in the research work of Ecoli O_ 157 :H7 molecular epidemiology. |
| |
| Keywords: | Ecoli O_(157):H_7 Genotype Optimization IS-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
