肉豆蔻挥发油对行为绝望抑郁小鼠的影响

目的 观察肉豆蔻挥发油对行为绝望抑郁小鼠的影响并探讨其作用机制.方法 健康雄性昆明种小鼠分为对照组、盐酸氟西汀组、肉豆蔻挥发油高、中、低剂量组,连续灌胃（ig）7d,末次给药0.5 h后通过悬尾实验、强迫游泳实验研究肉豆蔻挥发油的抗抑郁作用,采用酶联免疫法测定小鼠脑内5-羟色胺（5-HT）、多巴胺（DA）、去甲肾上腺素（NE）的含量.结果 与对照组（82.60±24.70）s]比较,肉豆蔻挥发油中剂量组小鼠悬尾不动的时间（54.40±15.87）s]显著降低,差异具有统计学意义（P＜0.05）;肉豆蔻挥发油中剂量组小鼠脑内5-HT、DA、NE的含量分别为（19.35±2.79） ng/ml、（12.16±0.71） pg/ml、（0.27±0.12）pg/ml,对照组小鼠脑内三种递质的含量分别为（14.95±4.83） ng/ml、（11.32±0.95） pg/ml、（0.20±0.11）ng/ml,中剂量组5-HT含量显著增高,差异具有统计学意义（P＜0.05）.结论 肉豆蔻挥发油有抗抑郁作用,可能与提高小鼠脑内5-HT、DA、NE的含量有关.

Effect of the volatile oil of Myristica fragrans Houtt on behaviors in despaire depressed mice

Objective To observe the antidepressant effect of the volatile oil of Myristica in mice and investigate its mechanism.Methods Healthy male Kunming mice were divided into control group,fluoxetine hydrochloride group,high,medium and low dose group,and all of the mice were gavaged for 7 days.The role of antidepressant on the mice were observed with tail suspension tests and forced swimming tests.Then serotonin（5-HT）,dopamine（DA） and norepinephrine （NE） in the brain of the mice were determined by enzyme-linked immunosorbent assay.Results Compared with the control group（（82.60 ± 24.70）s）,the medium dose group could shorten the immobility time of the tail suspension tests （（54.40± 15.87） s）,and showed statistical significance （P〈 0.05）.The content of 5-HT,DA,NE in the brain of the medium group were （19.35±2.79） ng/ml,（12.16±0.71）pg/ml,（0.27±0.12） ng/ml,and control group were （14.95±4.83） ng/ml,（11.32±0.95） pg/ml,（0.20±0.11） ng/ml.Compared with the control group,the content of 5-HT of the medium dose group was increased and showed statistical significance（P〈0.05）.Conclusion The volatile oil of Myristica fragrans Houtt has antidepressant effect,and may be related to raising the content of 5-HT,DA,NE in the brain of the mice,especially 5-HT.