多巴胺D4受体基因和5—HT2C受体基因与海洛因依赖

目的 探讨多巴胺D4受体基因外显子Ⅲ VNTR多态性和5－HT2C受体基因cys23/ser23多态性与海洛因依赖的关联。方法 采用聚合酶链式反应（PCR)技术，对102例海洛因依赖者和64例正常对照者的多巴胺D4受体基因外显子Ⅲ VNTR多态性进行检测，同时采用PCR结合限制性片段长度多态性（RFLP）分析技术，检测5－HT2C受体基因cys23/ser23多态性分布。结果 海洛因依赖组多巴胺D4受体基因外显子Ⅲ VNTR多态性的基因型和等位基因型的频率与对照组无显著差异，5－HT2C受体基因的ser23等位基因频率仅为0．9％。结论 未发现海洛因依赖与多巴胺D4受体基因外显子Ⅲ VNTR多态性相关联，在中国汉族人未发现5－HT2C受体基因cys23/ser23多态性。

Associations between heroin dependence and dopamine D4 and 5-HT2C receptor gem

Objective To explore the association between the dopamine D4 receptor gene exon III VNTR polymorphism and 23cys/23ser polymorphism of 5 HT2C receptor gene and heroin dependence. Method Genotype and allele frequencies of the dopamine D4 receptor gene exon III VNTR polymorphism and 23cys/23ser polymorphism of 5 HT2C receptor gene in 102 heroin addicts and 64 normal controls were examined by PCR and RLFP technique. Result The genotype and the allele frequency of dopamine D4 receptor gene exon III VNTR in heroin addicts had no difference from that in control group, the ser23 allele frequency of 5 HT2C is only 0.9%. Conclusion The dopamine D4 receptor gene exon III VNTR polymorphism has no association with heroin dependence. 23cys/23ser polymorphism of 5 HT2C receptor gene is not found in Hans of Chinese.