睡眠剥夺对大鼠脑5—羟色胺代谢及行为的影响

目的 探讨长时间睡眠剥夺对大鼠下脑脑和脑干５－羟色胺（５－ＨＴ）代谢及行为的影响。方法 用水上转盘睡眠剥夺模型对大鼠分别进行２４ｈ，４８ｈ，７２ｈ睡眠剥夺，观察睡眠剥夺后下丘脑，脑干两脑区５－ＨＴ代谢的变化，同时利用矿场实验观察大鼠主动行为的改变。结果 睡眠剥夺大鼠的矿场得分较非剥夺大鼠高（Ｐ〈０．０５），但随着时间的延长出现下降趋势，５－ＨＴ向５－羟吲哚乙酸（５－ＨＩＡＡ）转化在经过２４ｈ睡眠剥

The effects of prolonged sleep deprivation on brain serotonin metabolism and behavior in rats

Objective The effects of prolonged sleeep deprivation (SD) on hypothalamus and brain stem 5-hydroxytryptamine (5-HT)metabolism and behavior in rats were studied. Method Dish-over-watermodel was used in order to observe the effects of 24hr, 48hr and 72hr sleep deprivation on hypothalamus and brain stem 5-HT metabolism in Sprague-Dawley rats, and the active behavior of rats was also examined by open field test (OFT). Results The OFT scores weresignificantly increased after 24hr, 48hr and 72hr SD, but the scores after 72hr SD was lower than those after 24hr and 48hr SD(P <0. 01). The5-hydroxyindoleacetic acid/5-hydroxytryptamine (5-HIAA/5-HT) ratio were significantly increased after 24hr SD in the hypothalamus and brainstem, but the ratio were decreased after 48hr and 72hr SD. Conclusion The activity behavior in sleep deprived rats exhibted an pattern from activity to depression, which may he related to thtl 5-HT metabolic changes in hypothalamus and brain stem.