儿茶酚胺氧位甲基转移酶基因多态性与重性抑郁障碍的关联研究

目的探讨儿茶酚胺氧位甲基转移酶（eateehol一0-methyltransferase，COMT）基因Vall58Met（rs4680）多态性与中国汉族人群重性抑郁障碍（majordepressivedisorder，MDD）之间的关联。方法以250例MDD患者及300例健康对照作为研究对象，应用聚合酶链反应一限制性片段长度多态性（PCR-RFLP）技术测定研究对象的COMTVall58Met的基因型和等位基因频率分布。并采用t检验、Hardy—Weinberg平衡检验及x2检验进行统计学分析。结果病例组与对照组COMTVall58Met基因型及等位基因频率分布均差异有统计学意义（x2=20．268，12．611，P〈0．01），携带Met等位基因的人群患MDD的危险度是携带Val等位基因的1．725倍（OR=1．725，95％C／1．274～2．335）。结论COMTVall58Met基因多态性与MDD发病有关联，携带Met等位基因的人群患MDD的危险性增加。

The association study between polymorphisms of catechol-O-methyltransferase gene and major depressive disorder

Objective To explore the association between catechol-O-methyhransferase （COMT） Val158Met gene （rs4680） polymorphisms and major depressive disorder （MDD） in Han Chinese. Methods Genotypes and allele frequencies of Va1158Met polymorphisms of COMT gene were examined in 250 patients and 300 healthy controls by the method of polymerase chain reaction-restriction fragment length polymor- phism （PCR-RFLP）. t -test, Hardy Weinberg equilibrium and chi-square test were used to conduct statistical anal- ysis. Results There were significant differences between patients and control group in COMT Val158Met geno- types and allele frequency distribution （ ~2 = 20. 268,12. 611, P 〈 0.01 ）. Subjects with Met allele had a higher risk than those with Val allele to develop MDD （ OR= 1. 725,95% CI 1. 274-2.335）. Conclusion This research suggests that COMT Vall5$Met polymorphisms are associated with the onset of MDD. Met allele is a risk factor of MDD.