| 党参水溶性多糖含量测定方法的研究 |
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| 引用本文： | 李瑞燕,高建平.党参水溶性多糖含量测定方法的研究[J].长治医学院学报,2010,24(4). |
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| 作者姓名： | 李瑞燕 高建平 |
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| 作者单位： | 长治医学院药学系,046000;山西医科大学 |
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| 基金项目： | 山西省科技攻关项目，太原市科技局明星专项 |
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| 摘 要： | 目的:研究党参水溶性多糖的含量测定方法.方法:研究苯酚-硫酸法测定党参水溶性多糖时各因素对结果的影响,并对这些因素进行优化.结果:确定苯酚-硫酸法最佳反应条件为:在2 mL供试样品中加入1 mL苯酚,5 mL浓硫酸,沸水浴加热15 min,冷却至室温,在490 nm处测定吸光度值;同时进行了线性关系、精密度、重复性、稳定性及回收率等方法学考察,各项指标良好.结论:用苯酚-硫酸法结合3,5-二硝基水杨酸法来测定党参水溶性多糖的含量可用于党参多糖的质量控制.
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| 关 键 词： | 党参 水溶性多糖 含量测定 苯酚-硫酸法 |
The Study to Method of Content Determination of Radix Codonopsis Water-soluble Polysaccharides |
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| Li Ruiyan,Gao Jianping.The Study to Method of Content Determination of Radix Codonopsis Water-soluble Polysaccharides[J].Journal of Changzhi Medical College,2010,24(4). |
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| Authors: | Li Ruiyan Gao Jianping |
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| Institution: | Li Ruiyan,Gao Jianping Department of Pharmacology,Changzhi Medical College |
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| Abstract: | Objective:To investigate method of content determination of radix codonopsis water-soluble polysaccharides.Methods:The factors affacting the determination of polysaccharide content in radix codonopsis water-soluble polysaccharides with phenol-sulfuric acid method were optimized.Results: The optimal reaction conditions with phenol-sulfuric acid.methods:add 1 mL phenol and 5 mL concentrated sulfuric acid to the 2 mL samples tested,heating 15 minute in boiling water,then cooling to room temperature,the absorba... |
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| Keywords: | Radix Codonopsis Water-soluble Polysaccharides Content determination Phenol-sulfuric aid method |
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