| 毛蕊异黄酮对人黑色素瘤细胞A375增殖、迁移及侵袭的影响 |
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| 引用本文： | 刘彬,刘慰华,张竞之,孙学刚,黄桂琼,周迎春,刘世明.毛蕊异黄酮对人黑色素瘤细胞A375增殖、迁移及侵袭的影响[J].贵阳医学院学报,2014,39(2):162-166. |
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| 作者姓名： | 刘彬 刘慰华 张竞之 孙学刚 黄桂琼 周迎春 刘世明 |
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| 作者单位： | 刘彬(广州医科大学附属第二医院心血管疾病研究所,广东广州510260南方医科大学中医药学院,广东广州510515)；刘慰华(广州医科大学附属第二医院心血管疾病研究所,广东广州,510260)；张竞之(广州医科大学附属第二医院心血管疾病研究所,广东广州,510260)； 孙学刚 (南方医科大学中医药学院,广东广州,510515)； 黄桂琼 (南方医科大学中医药学院,广东广州,510515)； 周迎春 (南方医科大学中医药学院,广东广州,510515)； 刘世明(广州医科大学附属第二医院心血管疾病研究所,广东广州,510260)； |
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| 基金项目： | 国家自然科学基金(项目编号：81302892,81173459,81373575,81202841)广东省自然科学基金(项目编号：S2013040016226,S2013010014777)高等学校博士学科点专项科研基金(No.20124433110019):中国博士后基金(项目编号：2013M530363)广州医科大学博士启动项目(项目编号：2012C50) |
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| 摘 要： | 目的：研究毛蕊异黄酮对人黑色素瘤细胞A375增殖、迁移及侵袭的影响.方法：以人黑色素瘤细胞A375为研究对象,MTT法检测不同浓度的毛蕊异黄酮对A375细胞活性的影响,划痕实验检测A375细胞的迁移,Transwell小室检测A375细胞的侵袭作用;qPCR检测A375细胞中基质金属蛋白酶2（MMP-2）及基质金属蛋白酶9（MMP-9）表达水平.结果：毛蕊异黄酮在6.25 ～ 100 μmol/L浓度内不能抑制A375细胞的增殖,划痕实验及Transwell检测结果显示毛蕊异黄酮可显著抑制A375细胞的迁移及侵袭（P＜0.01）,qPCR结果显示毛蕊异黄酮能抑制A375细胞MMP-2及MMP-9的表达.结论：毛蕊异黄酮在小于100 μmol/L浓度时抑制A375细胞的迁移及侵袭,其机制可能与抑制MMP-2及MMP-9表达有关.
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| 关 键 词： | 黑色素瘤 基质金属蛋白酶 毛蕊异黄酮 A375细胞 迁移 侵袭 增殖 |
Effect of Calycosin on Proliferation,Migration, and Invasion of Human Melanoma Cell A375 in vitro |
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| LIU Bin LIU Weihua ZHANG Jingzhi SUN Xuegang HUANG Guiqiong,ZHOU Yingchun,LIU Shiming.Effect of Calycosin on Proliferation,Migration, and Invasion of Human Melanoma Cell A375 in vitro[J].Journal of Guiyang Medical College,2014,39(2):162-166. |
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| Authors: | LIU Bin LIU Weihua ZHANG Jingzhi SUN Xuegang HUANG Guiqiong ZHOU Yingchun LIU Shiming |
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| Institution: | 1. Institute of Cardiovascular Disease, the Second Aliated Hospital of Guangzhou Medical University, Guangzhou 510260, Guangdong, China ; 2. School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong , China) |
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| Abstract: | Objective： To investigate the effect of calycosin on proliferation, migration, and invasion of human melanoma cell A375. Method： Human melanoma cell A375 was chosen as study suhject; The cell viability was determined by MTT assay after treated with calycosin, and the migration and in- vasion of A375 cells were measured by scratch test and transwell assay; qPCR was used to detect the expression levels of matrix metalloproteinase-2 （MMP-2） and matrix metalloproteinase-9 （ MMP-9 ） in A375 cells. Result： Calycosin could not inhibit A375 cell proliferation in the concentration range of 6.25 -100 μmol/L. Calycosin can inhibit A375 cell migration and invasion, and qPCR results confirmed that calycosin could inhibit the mRNA expression of MMP-2 and MMP-9. Conclusion： When the concentration is less than 100 μmol/L, calycosin can attenuate A375 cell migration and invasion, and the mechanism may relate to inhibiting the mRNA expression of MMP-2 and MMP-9. |
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| Keywords: | melanoma matrix metalloproteinases calycosin A375 cells migration invasion pro- liferation |
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