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| 申克孢子丝菌的随机扩增DNA指纹分型 | |
| 引用本文： | 涂可正,曹煜,温海,喻茂娟,姚志,李志刚,柴建华,廖万清.申克孢子丝菌的随机扩增DNA指纹分型[J].贵阳医学院学报,2001,26(3):192-195. |
| 作者姓名： | 涂可正 曹煜 温海 喻茂娟 姚志 李志刚 柴建华 廖万清 |
| 作者单位： | 1. 贵阳医学院附院皮肤科, 2. 上海第二军医大学附属长征医院皮肤科, 3. 贵阳医学院科研处, 4. 复旦大学遗传所, |
| 基金项目： | 九五”军队重点资助项目 |
| 摘 要： | 目的：对申克孢子丝菌进行基因分型研究，探索申克孢子丝菌的基因分型标准。方法：用光镜对2株标准株，4株环境分离株和24株临床分离菌株进行形态特征观察，再用随机扩增DNA指纹方法(RAPD)对DNA进行PCR扩增，根据扩增产物的电泳带型来分析DNA多态性。结果：30株菌株镜下可见2种形态结构；30株申克孢子丝菌的DNA指纹带型不同；多引物聚类分析表明，3条引物可将30株不同地区来源申克孢子丝菌分作11个型。结论：(1)在我国申克孢子丝菌中存在2种不同的形态结构。(2)申克孢子丝菌中存在不同的基因型。(3)RAPD法用于申克孢子丝菌菌株鉴定是一种快速、方便、可行的方法。 |
| 关 键 词： | 基因 真菌 DNA指纹法 申克孢子丝菌 |
| 文章编号： | 1000-2707(2001)03-0192-03 |
| 修稿时间： | 2001年4月2日 |
Typing of Sporothrix schenckii by Random Amplification of DNA Fingerprinting |
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| TU Ke zheng ,CAO Yu ,WEN Hai ,YU Mao juan ,YAO Zhi rong ,LI Zhi gang ,CHAI Jian hua ,LIAO Wan qing.Typing of Sporothrix schenckii by Random Amplification of DNA Fingerprinting[J].Journal of Guiyang Medical College,2001,26(3):192-195. | |
| Authors: | TU Ke zheng CAO Yu WEN Hai YU Mao juan YAO Zhi rong LI Zhi gang CHAI Jian hua LIAO Wan qing |
| Institution: | TU Ke zheng 1,CAO Yu 1,WEN Hai 2,YU Mao juan 3,YAO Zhi rong 2,LI Zhi gang 2,CHAI Jian hua 4,LIAO Wan qing 2 |
| Abstract: | Objective:To genotype Sporothrix schenckii and find out a suitable genotyping standard for S.schenckii.Methods:The morphic characters of 2 standard strains,4 environmental and 24 clinical isolates in EMS were observed,then by using the RAPD method,DNA from all strains were amplified and analysised employing produce of DNA fingerprinting.Results:30 strains had two different forms in EMS with different patterns in DNA fingerprinting. The multi primer clustering showed that 30 isolates of S.schenckii from different geographical locations could be classified into 11 types by using 3 primers. Conclusions:(1) S.sckenckii has two different forms in China.(2)S.sckenckii has different genotyping.(3)RAPD is a rapid,simple and feasible method for identifying S.sckenckii. |
| Keywords: | genes fungal DNA fingerprinting Sporothrix schenckii |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |