燃煤型氟中毒对大鼠睾丸生精细胞凋亡的影响

目的：观察燃煤型氟中毒对大鼠睾丸生精细胞凋亡的影响。方法：5月龄SD雄性大鼠18只,按体质量随机分为染氟组、染氟＋大豆组和对照组,各组大鼠自由饮用自来水（含氟〈0.5 mg/L）;各染氟组采用含氟量为69.33 mg/kg的煤烘玉米饲食,染氟＋大豆组的大豆重量比为20%,对照组采用含氟为9.37 mg/kg的常食玉米饲食,实验期间动物自由进食、进水;染氟第90天,处死大鼠,使用H-E染色法和TUNEL方法对大鼠睾丸切片进行染色,观察细胞凋亡情况,结果：染氟组生精细胞凋亡个数高于对照组（278.17±25.47）个,（129±21.02）个,P〈0.05];染氟＋大豆组生精细胞凋亡个数高于对照组（141.5±5.24）个,（129±21.02）个,P〈0.05];染氟＋大豆组生精细胞凋亡数低于染氟组（141.5±5.24）个,（278.17±25.47）个],P〈0.05。结论：燃煤型氟中毒可造成大鼠生精细胞凋亡增加,增加营养后可有效减少氟中毒造成的生精细胞的凋亡。

Effect of Coal-Burning Caused Fluorosis on Apoptosis of Spermatogenic Cells in Male Rats

Objective： To observe the effect of coal-burning caused fluorosis on apoptosis of sperma-togenic cells in adult male rats.Methods： Eighteen healthy SD adult male rats aged 6 months were randomly divided into 3 groups（6 in each group）： Control group（group C,fed with normal maize containing 9.37 mg/kg of fluorine）,fluoride group（group F,fed with coal baked maize containing 69.33 mg/kg of fluoride）,and fluoride plus soybean group（group S,fed with maize as group F plus 20% soybean）.All the rats drank tap water in which sodium fluoride concentration was less than 0.5mg.Rats in 3 groups were killed in 90 days,and morphological changes of their testis were observed under light microscope after H-E staining.The number of apoptosis cells（NAC） in testis were detected by TUNEL assay.Results： NACs of group F（278.17±25.47） and group S（141.5±5.24） were bigger than that of group C（129±21.02）（P〈0.05）,and NAC of group F was bigger than that of group S（P〈0.05）.Conclusions： Coal-burning caused fluorosis can lead to high apoptosis of spermatogenic cells of rats,and adding nutrition can effectively reduce cellular apoptosis.