细胞因子对HIV/AIDS患者的免疫调节作用

目的 探讨多种细胞因子对高效抗反转录病毒治疗（HAART）的HIV/AIDS患者的免疫调节作用.方法 确诊HIV感染者或AIDS患者40例,CD4＋T细胞数均＜350/μl,接受HAART治疗,并分别于HAART治疗的0、12及24个月随访备血,健康对照组30例.ELISA检测血清和上清液细胞因子（IL-12、IL-21、IP-10和IFN-γ）,流式细胞术检测CD4＋T细胞、CD8＋T细胞、CD4＋CD25＋FOXP3 Treg细胞.结果 未经抗病毒治疗的HIV/AIDS患者外周血中IL-12、IL-21和IFN-γ水平显著低于正常人,CD4＋T细胞明显降低,HAART治疗后均不同程度升高;而IP-10、CD8＋T细胞、CD4＋CD25＋FOXP3 Treg细胞显著高于正常人,HAART治疗后水平下降.CD4＋T、CD8＋T、CD4＋CD25＋FOXP3 Treg与各细胞因子水平有一定的相关性.结论 细胞因子可能和HIV/AIDS感染的致病机制相关,并可能参与了抗HIV病毒免疫重建和病毒控制过程.

Regulation of cytokines during in HIV/AIDS patients

Objective To investigate the regulation of some cytokines in human immunodeflciency virus （HIV）/acquired im- munodeficiency syndrome （AIDS） patients and their dynamic changes during 24 months of highly active antiretroviral therapy （HAART）. Methods Forty Chinese HIV-1/AIDS adults, while their CD4^＋ T cells were less than 350/ram^3 in peripheral blood, were accepted HAART. Vein blood was obtained from each patient at baseline （month 0）, month 12 and 24 of HAART initiation and control groups. CD4^＋, CD8^＋, and CD4^＋ CD25^＋ FOXP3 Treg ceils were detected with flow cytometry. HIV RNA level was tested by real-time fluorescent quantitative polymerase chain reaction （RT-PCR）. Meanwhile, enzyme linked immunosorbent assay （ELISA） was used to detect the serum levels of interleukin 12 （IL-12）, IL-21, interferon-inducible protein 10 （IP-10）, and interferon gamma （IFN-3,）. Results IL-12, IL-21, IFN-γ, and CD4^4 T cells showed significantly lower, while the IP-10 and CD8^＋ T, CD4^＋ CD25^＋ FOXP3 Treg cells significant higher in HIV infected patients than normal persons. After HAART initiation, IL-12, IL-21, and IFN-γ, levels were increased gradually, and were correlated positively with CD4^＋ T cell counts; while, IP-10 levels and CD8^＋ T, CD4^＋ CD25^＋ FOXP3 Treg cells were decreased gradually. CD4^＋ T, CD8^＋ T, and CD4^＋ CD25^＋ FOXP3 Treg cells were relative to some cyto- kines. Conclusions This study found a close relationships between cytokine levels and CD4 cell counts during 24-month HAART, and these cytokines might play appropriate roles in AIDS pathogenesis, and antiviral procedure and immune constitution.