汉坦病毒感染BALB/c小鼠组织中特异性抗原及病毒RNA的检测 |
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引用本文: | 徐永妮,胡至察,程林峰,张芳琳.汉坦病毒感染BALB/c小鼠组织中特异性抗原及病毒RNA的检测[J].当代医师,2013(11):1441-1445. |
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作者姓名: | 徐永妮 胡至察 程林峰 张芳琳 |
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作者单位: | [1]第四军医大学微生物学与病原生物学教研室,西安710032 [2]解放军第三二三医院检验科,西安710032 |
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基金项目: | 基金项目:国家自然科学基金(31070810;81001344) |
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摘 要: | 目的检测汉坦病毒感染BALB/c小鼠组织中特异性抗原及病毒RNA,建立汉坦病毒感染动物的评价体系。方法将1000LD50的汉坦病毒悬液经肌肉注射感染BALB/c小鼠,在感染后的第3天,分别取小鼠的心、肝、脾、肺、肾、脑等组织研磨后制成病毒悬液,以ELISA法和qRT—PCR法分别检测各组织中的汉坦病毒特异性抗原及病毒RNA。结果BALB/c小鼠感染汉坦病毒后短期内在脑和肝组织中可以检测到大量汉坦病毒特异性抗原以及病毒RNA,而心、脾、肺、肾组织中未检测到特异性抗原及病毒RNA。结论实验结果为建立汉坦病毒感染动物模型的评价体系提供了参考依据。
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关 键 词: | 汉坦病毒感染 小鼠 近交BALB C 抗原 病毒 RNA 病毒 |
Detection of the specific antigens and viral RNA in the tissues of the BALB/c mice infected with hantavirus |
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Authors: | XU Yong-ni HU Zhi-cha CHENG Lin-feng ZHANG Fang-lin |
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Institution: | .Department of Microbiology and Pathogenic Biology, Fourth Military Medical University, Xi'an 710032, China |
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Abstract: | Objective To establish an evaluation system about animals infected with hantavirus, an observation of the BALB/c mice infected with hantavirus was made. Methods BALB/c mice were in- fected with hantavirus by intramuscular injection with stock solution . The specific antigen from BALB/c mice tissues after 3 days was detected with enzyme-linked immunosorbent assay (ELISA) and viral RNA with real-time polymerase chain reaction (RT-PCR). Results Within a short term, the specific antigen and viral RNA were detected from the brain and liver at day 3 after infection, but not be detected from the heart, spleen, lung, and kidney samples. Conclusions' The results provided ones with some information on animals infected with hantavirus. |
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Keywords: | Hantavirus infections Mice inbred BALB C Antigens viral RNA viral |
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