角蛋白34βE12在食管鳞状细胞癌中的表达及其意义

【摘要】目的研究角蛋白34βE12在不同食管组织中的表达及其与临床生物学行为的关系，进而探讨其在食管癌发生、发展中的作用机制。方法采用免疫组织化学方法分析252例食管鳞状细胞癌、66例食管原位癌及106例癌旁正常食管黏膜组织中角蛋白34βE12的表达情况，并根据临床资料评价其表达与生物学行为的关系；另收集其中60例新鲜食管癌组织及其癌旁正常食管组织，采用Western印迹法测定组织中角蛋白34βE12的表达情况。结果（1）角蛋白34βE12在食管鳞癌、原位癌及癌旁正常食管组织中的阳性表达率分别为85．7％、54．5％和25．7％，差异均具有统计学意义（P〈0．01）。（2）角蛋白34βE12在食管鳞癌临床I期、Ⅱ期及Ⅲ期的表达率分别为76．5％，84．7％和96．3％，癌组织的表达强度随着肿瘤临床分期的升高而逐渐增强，差异具有统计学意义（P：0．038）；淋巴结转移组角蛋白34βE12的表达明显高于无淋巴结转移组（P〈0．01）。（3）Western印迹法结果进一步证实食管鳞癌组织的角蛋白34βE12的表达明显高于癌旁正常食管组织（P〈0．01）。结论角蛋白3413E12的高表达可能参与了食管癌的发生、发展过程；角蛋白34βE12的表达与食管癌临床分期有一定的关系，其很可能成为潜在的治疗靶点及判断食管癌预后的有价值的肿瘤标记物。

The expression and significance of cytokeratin 34βE12 in esophageal squamous cell carcinoma

Abstract] Objective To investigate the expression of cytokeratin 34βE12 in esophageal squamous cell carcinoma （ESCC）, and its mechanism of action in the process of occurrence and development of an ESCC. Methods Immunohistochemistry was used to analyze the expression of cytokeratin 34βE12 in 252 ESCC patients, 66 patients with esophageal carcinoma in situ, and 106 patients with adjacent normal esoph- ageal mucosa before the relationship between its expression and biological behavior was evaluated on the ba- sis of complete clinical information. In addition, Western blotting was used to determine the expression of cytokeratin 34βE12 in 60 patients with esophageal cancer and adjacent normal esophageal tissues. Results （1）The positive rate of caveolin-I in ESCC, carcinoma in situ, and adjacent normal tissues was 85.7%, 54. 5%, and 25. 7%, respectively. The difference between them was statistically significant （ P 〈0. 01 ）. （2）The positive rate of cytokeratin 34βE12 in stages I, II, III of ESCC was 76. 5%, 84. 7%, and 96. 3%, respectively. The expression intensity of cytokeratin 3413E12 in carcinoma tissue was gradually increased with the advance of clinical stages with a statistically significant difference （ P =0. 038）. The positive rate of cytokeratin 3413E12 with group of lymph node metastasis was significantly higher than those without lymph node metastasis （ P 〈 0. 01 ）. （3）Western blotting results further confirmed that the expression of cytokeratin 34βE12 in ESCC was significantly higher than that in adjacent normal esophageal tissue （ P 〈0. 01 ）. Con- clusions The high expression of caveolin-1 might be involved in the occurrence and development of esoph-ageal cancer. The expression of cytokeratin 34βE12 was correlated with the clinical stage of esophageal cancer, cytokeratin 34βE12 was a potential therapeutic target and a valuable prognostic indicator of esopha- geal cancer progression.