孕早期外周血VEGF165b降低对预测子痫前期的作用研究

目的:分析血管内皮生长因子165b(VEGF165b)在子痫前期(PE)患者血清中的表达,探讨孕早期外周血VEGF165b作为预测PE发生的生物学指标的临床意义。方法:酶联免疫吸附试验(ELISA)和竞争性酶免疫分析(cEIA)分别检测47例PE患者和42例无高血压正常孕妇、40例未怀孕正常妇女血清中VEGF165b、VEGF的含量。ROC曲线分析孕12周VEGF165b预测PE发生的灵敏度。结果:孕12周,正常孕妇组血清VEGF165b含量(4.49±1.57)ng.ml-1]与未怀孕组(0.42±0.18)ng.ml-1]相比显著升高(P<0.01),发展成PE患者的外周血VEGF165b含量(1.19±0.50)ng.ml-1]与正常孕妇组相比显著降低(P<0.01);ROC曲线显示孕12周VEGF165b预测PE发生具有较高的灵敏度(89.8%)。结论:孕早期母体外周血VEGF165b显著降低可预测PE发生。

Effects of low VEGF165b on predicting increased risk of pre-eclampsia

Objective:To evaluate the signification of vascular endothelial growth factor165b(VEGF165b) for predicting increased risk of pre-eclampsia(PE),and to detect the expression of VEGF165b in plasma of PE patients.Methods: Forty-seven patients with PE,42 normotensive pregnant women and 40 nonpregnant women were recruited in this study.The concentration of VEGF165b and VEGF in plasma of all women were analyzed with the methods of ELISA and cEIA respectively.Results: At 12 weeks of gestation,the VEGF165b concentration was significantly up-regulated in plasma from normotensive group (4.49±1.57)ng·ml-1] compared with non-pregnant women (0.42±0.18) ng·ml-1,P<0.01].In contrast,in patients who later developed pre-eclampsia,VEGF165b levels (1.19±0.50)ng·ml-1] were significantly lower than in the normotensive group,but were greater than nonpregnant women.The serum concentrations of total VEGF were increased in the patients with PE compared to the women with normotensive group (35.6±5.7) vs(29.3±4.7) ng·ml-1,P<0.01].ROC curve indicate that low VEGF165b in first trimester plasma may sensitively predict the risk of pre-eclampsia.Conclusion: Plasma VEGF165b levels may be a clinically useful first trimester plasma marker for increased risk of pre-eclampsia.