低分子肝素柔性纳米脂质体的研究

目的 :研制用于透皮吸收的低分子肝素柔性纳米脂质体制剂。方法 :用超声波分散法制备低分子肝素柔性纳米脂质体 ,以正交实验设计确定最佳制备工艺 ;电镜观察其形态及粒径分布 ,以Franz扩散池法测定其体外经皮渗透动力学 ,以小鼠用药前后血液凝固时间的变化研究其促透作用。结果 :低分子肝素柔性纳米脂质体在电镜下为圆形或椭圆形 ,平均粒径为 85 .4nm ,包封率为 35 .2 % ;其体外经皮渗透符合Fick’s扩散方程 ,小鼠用药后血液凝固时间显著延长。结论 :柔性纳米脂质体对低分子肝素有良好的透皮促进作用

Study on flexible nanoliposomes of low molecule weight heparin

Objective:The purpose of the study was to dvelop a preparation of flexible nanoliposomes of low molecule weight heparin (LMWH). Methods: Flexible nanoliposomes were prepared through ultrasonication ,and the shape and diameter distribution of flexible nanoliposomes was observed under electronic microscope. An optimal technique was chosen with orthogonal design ,and the kinetics penetrating through mouse skin in vitro was tested by Franz diffusion cell. The penetration enhancing effect was determined by the changes of mouse blood coagulation time. Results: The flexible nanoliposomes LMWH ohtained were globular or elliptic with, the mean diameter of about 85.4nm, and the encapsulated ratio of 35.2% and the penetration through mouse skin in vitro followed Fick's diffusion equation . Mouse blood coagulation time was significantly prolonged. Conclusion: Flexible nanoliposomes have a significant skin penetration enhancing effect on low molecule weight heparin.