肝细胞生长因子mRNA在牵张成骨过程中的表达

目的 探讨肝细胞生长因子(HGF)mRNA在兔下颌骨牵张成骨(DO)过程中的表达。方法 选取新西兰大白兔40只分为牵张组与骨折对照组，每组20只，牵张组行两侧下颌骨切开术，经7?d间歇期后以0.5mm/12h的速度牵张，7d后固定，骨折对照组不牵张，分别于术后第1、2、3、5、7周末各处死4只动物。采用HE染色和原位杂交进行组织学分析并检测HGF的表达。结果 HGFmRNA在术后1周开始出现少量表达，位于牵张间隙内炎性细胞的细胞浆，术后3周时阳性表达强度达到峰值，牵张组与骨折对照组之间阳性表达差异有统计学意义(P＜0.05)，术后5周时基本检测不到目的基因的阳性表达，牵张组与骨折对照组之间差异无统计学意义(P＞0.05)。结论 DO过程中HGFmRNA在机体内出现阳性表达，且牵张力可促进该因子的阳性表达强度。

Expression of hepatocyte growth factor mRNA in distraction osteogenesis

Objective To explore the expression of hepatocyte growth factor(HGF) mRNA in the course of distraction osteogenesis(DO).Methods Forty New Zealand white rabbits were divided into two groups: the distraction group and the control group.In the distraction group,the stretch rate was set as 0.5 mm/12 h after a 7-day interval and was fixed for 7days.The control group was free of distraction and four animals of each group were sacrificed at the end of week 1,2,3,5 and 7 after operations.Expression of HGFmRNA was determined by hematoxylin-eosin staining(HE staining) and in situ hybridization(ISH stain).Results HGFmRNA was mainly expressed in inflammatory cells at the end of the intermittent stage.Positive staining was seen at the end of the distraction stage in vascular endothelial cells and mesenchymal cells.The peak intensity of expression was found at the end of week 3 in the distraction group(P<0.05) and there was little expression at week 7.No remarkable differences were found between the two groups at the end of the fixation(P>0.05).Conclusions The present study shows that endogenous HGF may play an important role on cell proliferation,differentiation and vessel formation.Positive expression of HGFmRNA appears in the course of DO and that could be induced by distraction force.