Ⅱ型糖尿病患者房水中VEGF和IL-6含量的检测

目的：检测Ⅱ型糖尿病患者眼房水中血管内皮生长因子（vascular endothelial growth factor，VEGF）和白细胞介素-6（interleukin-6，IL-6）的含量，并探讨其临床意义。方法:采用双抗体夹心酶联免疫吸附（ELISA）法测定64例Ⅱ型糖尿病患者房水中VEGF和IL-6的含量，根据散瞳眼底检查和眼底荧光素血管造影检查后，实验组分为：无糖尿病视网膜病变组（NDR）21例、单纯型糖尿病性视网膜病变组（BDR）25例、增生型糖尿病性视网膜病变组（PDR）18例，对照组为健康的老年性白内障患者20例。结果：NDR组、BDR组、PDR组的房水VEGF含量分别为(220.27±25.69)pg/ml、（284.31±58.92）pg/ml、（459.07±90.90）pg/ml, IL－6 含量分别为(154.09±32.76)pg/ml、（221.81±60.52）pg/ml、（387.95±90.22）pg/ml，对照组房水VEGF含量为（135.50±25.69）pg/ml、IL-6含量为（78.71±20.37）pg/ml，对照组与实验组比较差异均有统计学意义（F=108.72,P＜0.01;F=102.532,P＜0.01）。 4组房水VEGF和IL 6含量有明显增加的趋势。房水中的VEGF含量与相应的IL 6含量有相关性（r=0.995, P＜0.01）；房水中VEGF、IL-6含量与糖尿病患者的病程及糖尿病视网膜病变的严重程度有相关性（r=0.722,P＜0.01; r=0.716,P＜0.01）、（r=0.869,P＜0.01;r=0.865, P＜0.01）。结论：VEGF、IL-6在糖尿病视网膜病变的形成过程中有重要作用，且两者之间有相关性。

Level of VEGF and IL-6 in the aqueous humor of Ⅱ diabetic patients

Objective: To explore the role of VEGF and IL-6 in the aqueous humor in the pathogenesis of diabetic retinopathy (DR) . Methods: Aqueous humor samples were collected from diabetic patients with cataracts or without retinopathy,and aqueous humor samples from non-diabetic patients with cataracts were chosen as controls. The content of VEGF in the aqueous humor was determined by using the ELISA technique. Results: The level of VEGF in the aqueous humor samples of diabetic patients without retinopathy(NDR), background diabetic retinopathy (BDR) and proliferative diabetic retinopathy (PDR) were（220.27±25.69）pg/ml, （284.31±58.92）pg/ml and （459.07±90.90）pg/ml. The level of IL-6 in the aqueous humor from NDR, BDR and PDR were（154.09±32.76）pg/ml,（ 221.81±60.52 ）pg/ml and （387.95±90.22）pg/ml． The levels of VEGF and IL-6 in the aqueous humour from the control group were（135.50±25.69）pg/ml,（ 78.71±20.37）pg/ml. Compared with the control, the level of VEGF and IL 6 in the aqueous humor of diabetic patients increased significantly (P＜0.01). The level of VEGF and IL-6 in the aqueous of the NDR, BDR and PDR also increased significantly (P＜0.01). The level of VEGF significantly correlated with the level of IL-6 in the aqueous humor（r=0.995, P＜0.01）. The level of VEGF and IL-6 in the aqueous humor also significantly correlated with the severity and prolongation of DR（r=0.869,　P＜0.01; r=0.865,　P＜0.01）. Conclusion: VEGF and IL-6 play active roles in the generation and development of DR, and the level of VEGF in the aqueous humor closely correlates with the level of IL-6 in the aqueous humour of diabetic patients.