拉莫三嗪对癫痫大鼠的脑电图及Bax、Bcl-2表达的影响

目的：探讨拉莫三嗪对青霉素致痫大鼠行为和脑电图的影响及其对海马神经元的保护作用。方法：观察青霉素致痫组、拉莫三嗪组、丙戊酸钠组大鼠痫性发作，并记录其脑电图。采用免疫组化染色法观察Bcl-2、Bax蛋白的表达。结果：拉莫三嗪组与丙戊酸钠组脑电痫样放电减少，两组比较，癫痫发作潜伏期和发作持续时间有统计学意义（P＜0.01）。拉莫三嗪组、丙戊酸钠组较青霉素致痫组Bcl-2细胞增多，Bax细胞减少（P＜0.01）。拉莫三嗪组较丙戍酸纳组Bcl-2细胞增多（P＜0.01）。结论：拉莫三嗪抗癫痫疗效优于丙戊酸钠。拉莫三嗪对青霉素致痫大鼠模型海马神经元有保护作用。

Effects of lamotrigine on the electroencephalogram and the expression of Bax and Bcl-2 in epileptic rats

Objective: To explore the effect of lamotrigine(LTG) on the behavior and electroencephalogram(EEG) of epileptic rats induced by penicillin and to explore the protective effect of LTG on the neurons of the hippocampus.Methods: Seizures in the group of epileptic rats induced by penicillin,the group of the LTG,and the group of the sodium valproate were measured,and the EEG was recorded for the groups.Immunohistochemical staining was used to observe the expression of Bcl-2 and Bax.Results: The epileptiform discharges were decreased in the group of the LTG and the group of the sodium valproate.The latencies and durations of seizures produced a significant difference between the group of LTG and the group of sodium valproate(P<0.01);Compared with the epileptic group induced by penicillin,Bcl-2 positive cells increased and Bax positive cells decreased in the group of LTG and the group of sodium valproate(P<0.01).Compared with the group of sodium valproate,Bcl-2 positive cells in the group of LTG increased(P<0.01).Conclusions: LTG is better than sodium valproate in anti-epilepsy,and it can protect the hippocampal neurons in epileptic rats induced by penicillin.