非小细胞肺癌区域淋巴结中肺组织特异性基因的表达与预后的关系

目的：探讨非小细胞肺癌（non-small-cell lung cancer，NSCLC）区域淋巴结人肺组织特异性基因（lunx mRNA）的表达与预后的关系。方法：应用SYBR GreenⅠ嵌合荧光法进行逆转录聚合酶链反应（real-time RT-PCR）用以检测74例NSCLC手术切除区域淋巴结Lunx mRNA的表达，对入选患者进行为期3年的随访观察，并对6个可能影响NSCLC患者预后的因素进行Cox回归模型分析。结果：手术切除区域淋巴结Lunx表达率与年龄、营养指数、3年内的复发或转移率之间有相关性（P＜0.01, P＜0.01, P＜0.05），而患者的性别、肺癌病理类型、分期与手术切除区域淋巴结Lunx表达率之间无相关性（P＞0.05）；NSCLC患者的生存时间与术后辅助治疗及与Lunx表达有统计学意义（P＜0.05, P＜0.01）。结论：NSCLC患者Lunx mRNA表达与其生存时间显著相关, 提示Lunx mRNA可作为NSCLC淋巴微转移的分子标志物及监测患者3年预后的指标之一；同时术后辅助治疗是延长患者生存时间的一项重要措施；荧光实时RT-PCR相对定量方法可应用于NSCLC淋巴微转移的检测，该方法可能有助于早期肺癌转移的诊断。

mRNA expression of lunx in regional lymph nodes of non-small cell lung cancer and its correlation with prognosis

Objective： To explore the mRNA expression of lunx in regional lymph nodes of non-small cell lung cancer (NSCLC) and its correlation with prognosis. Methods SYBR greenⅠreal-time RT-PCR was used to determine the lunx mRNA level in 153 regional lymph nodes obtained from 75 patients with NSCLC. All patients had been followed up for 3 years. Six factors that may influence the survival rate were analyzed by a Cox regression model. Results： Expressions of lunx in regional lymph nodes had a statistical correlation with age, nutritional index, relapse within 3 years and metastasis(P＜0.01, P＜0.01, and P＜0.05) but had no correlation with gender, tumor types and pathological grades（P＞0.05). There were statistical significances between the survival of NSCLC patients and post-operative adjuvant therapy and expression of lunx (P＜0.05 and P＜0.01). Conclusions： The expression of Lunx in regional lymph nodes closely correlates with the survival of NSCLC patients and it may be a target gene for detection of micrometastasis from NSCLC and a marker for detection in 3 years prognosis. Quantitative real-time RT-PCR is helpful in early diagnosis of micrometastasis of the lymph node in patients with NSCLC.