米非司酮对人早孕绒毛组织核转录因子-κB表达的影响

目的 探讨米非司酮对人早孕绒毛组织中核转录因子-κB(nf-κB)表达的影响.方法 人工流产组早孕绒毛18例和药物流产组早孕绒毛22例,应用逆转录聚合酶链反应(RT-PCR)和免疫组织化学技术分别测定两组早孕绒毛组织中NF-κB蛋白和mRNA的分布与含量.结果 药物流产组早孕绒毛组织中NF-κB mRNA表达量与人工流产组相比下调明显(P<0.01);药物流产组早孕绒毛细胞滋养层细胞中NF-κB蛋白表达与人工流产组相比明显下调(P<0.05).结论 米非司酮引起早孕绒毛NF-κB表达降低,可能是米非司酮终止早孕的机理之一.

Effect of mifepristone on expression of nuclear factor-κB in human villi during early pregnancy

Objective  To probe into the effect of mifepirstone on expression of nuclear factor-κB（NF-κB） in human villi during the early pregnancy. Methods  Expression of NF-κB was detected in human villi of 18 surgical abortion women and 22 medical abortion women by RT-PCR and immunohistochemical analysis. Results  Expression of NF-κB mRNA in villi of the medical abortion group was significantly weaker when compared with that of the surgical abortion group(P＜0.01)，and expression of NF-κB protein in cytotrophoblasts of the medical abortion group was significantly weaker when compared with that of the surgical abortion group(P＜0.05). Conclusions  Decreased expression of NF-κB in human villi induced by mifepristone may be one of the mechanisms for terminating early pregnancy.