| 利用显色底物CBZ-GLY-PRO-ARG-pNA测定血浆纤溶酶原活性 |
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| 引用本文： | 徐从高,朱嫒嫒,王勤,张锑,李同义,张茂宏.利用显色底物CBZ-GLY-PRO-ARG-pNA测定血浆纤溶酶原活性[J].山东大学学报(医学版),1992(2). |
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| 作者姓名： | 徐从高 朱嫒嫒 王勤 张锑 李同义 张茂宏 |
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| 作者单位： | 山东医科大学血液病研究室，山东医科大学血液病研究室，山东医科大学血液病研究室，山东医科大学血液病研究室，山东医科大学血液病研究室，山东医科大学血液病研究室 |
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| 基金项目： | 国家自然科学基金(3860796) |
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| 摘 要： | 用国产显色三肽CBZ-gly-pro-arg-pNA为底物测定血浆纤溶酶原(plg)活性,选择链激酶(SK)为plg激活物,向待测标本中加入足量的SK与plg形成具有纤溶酶活性的复合物,对实验系统中的显色底物产生酰胺分解作用,裂解出显色集团对硝基苯胺。血浆中plg活性与显色反应呈正相关。作者对实验的最佳条件和影响因素作了讨论。用本法测定60例健康者plg活性,提出正常参考值范围为100.00±8.69%(■±S)。
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| 关 键 词： | 血纤维蛋白溶酶原类 酰胺分解法 生色化合物 链激酶类 |
DETERMINATION OF PLASMA PLASMINOGEN ACTIVITY USING CBZ-GLY-PRO-ARG-pNA AS CHROMOGENIC SUBSTRATE |
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| Xu Conggao,et al Hematology Research Laboratory of Shandong Medical University.DETERMINATION OF PLASMA PLASMINOGEN ACTIVITY USING CBZ-GLY-PRO-ARG-pNA AS CHROMOGENIC SUBSTRATE[J].Journal of Shandong University:Health Sciences,1992(2). |
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| Authors: | Xu Conggao Hematology Research Laboratory of Shandong Medical University |
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| Abstract: | Presented in this paper is an amidolytic method for deter-mination of plasma plasminogen activity using a domestically made chro-mogenic tripeptides CBZ-gly-pro-arg-pNA as substrate.Streptokinase in excess was chosen as plasminogen activator and incubated with plasma in the presence of the substrate.The chromogenic moiety pNA cleft from the sub-strate was spectrophotometrically measured,representing plasminogen acti-vity in plasma.A strict linear correlation was demostrated between color-metry and plasminogen activity in plasma ranging from 40%-120%.The optimal conditions and influential factors of the assay were investigated and delineated. |
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| Keywords: | Plasminogen Amidolytic method Chromogenic compounds Streptokinase |
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