2型糖尿病发病过程中外周血miR-34a的表达水平及其临床意义

目的 研究外周血中糖尿病相关的microRNA (miRNA) miR-34a 在2型糖尿病（T2D）发病过程中的表达水平及临床意义。方法 63例研究对象中18例为初发T2D患者（n-T2D组），19例为T2D前期患者（pre-diabetes组），26例为糖耐量正常的T2D易感个体（s-NGT组）。所有诊断均经口服葡萄糖耐量试验（OGTT）证实。以荧光定量实时RT-PCR法检测其血清miR-34a的表达水平。结果 s-NGT组、pre-diabetes组和n-T2D组血清样本中miR-34a水平呈现序贯上升的趋势，而n-T2D组显著高于s-NGT组和pre-diabetes组（P＜0.05）；s-NGT组与pre-diabetes组间差异无统计学意义（P＞0.05）。结论 外周血miR-34a在T2D发病过程中具有重要意义，但在T2D前期阶段尚未发生明显改变。

Expression and clinical significance of peripheral miR-34a during the onset of type 2 diabetes

Objective To study expression and clinical significance of peripheral diabetes-related microRNA(miRNA),miR-34a,during the onset of type 2 diabetes(T2D).Methods 63 subjects were recruited,including 18 cases of newly diagnosed T2D(n-T2D),19 cases of pre-diabetes and 26 T2D-susceptible with normal glucose tolerance(s-NGT).All diagnoses were confirmed by a 2-hour oral glucose tolerance test(OGTT).Serum miR-34a levels were determined by real-time PCR(RT-PCR).Results Serum miR-34a levels showed a stepwise increas...