大蒜素对高半胱氨酸所致内皮细胞损伤的保护作用

目的：研究大蒜素对高半胱氨酸所致内皮细胞损伤的保护作用。方法：将培养的内皮细胞分成高半胱氨酸组（模型对照组）、大蒜素大剂量组、大蒜素中剂量组、大蒜素小剂量组、空白对照组5组,分别加不同的培养液，并于加药后的12、24、36?h取出上清液测乳酸脱氢酶（LDH）；另外采用MTT比色法观察细胞的存活率。结果：大蒜素小、中剂量组显示出对内皮细胞的保护作用：大蒜素小、中剂量组与模型对照组比较均能明显降低LDH含量（P＜0.05）；大蒜素大剂量组在36?h拮抗Hcy损害作用降低，跟模型对照组比较差异无统计学意义（P＞0.05）。这一结果跟MTT测试结果吻合：小、中剂量的大蒜素组光吸收值A较模型对照组明显升高（P＜0.01），大蒜素大剂量组A值跟模型对照组比较差异无统计学意义（P＞0.05）。结论：大蒜素小、中剂量可明显降低高半胱氨酸对内皮细胞的损伤，有效防治高半胱氨酸血症。

Protective effect of allicin on homocysteine induced endothelial cell injury

Objective: To explore the reverting effect of allicin on homocysteine induced endothelial cell injury.Methods: Cultural endothelial cells were divided into 5 groups: the homocysteine group,the high dose allicin group,the middle dose allicin group,the low dose allicin group and the control group.Different cultural solutions were added,12,24 and 36 hours later,and LDH separated from the upper fluid were determined.The MTT colorimetric method was used to observe the survival of endothelial cells.Results: The low and the middle dose allicin groups had an antagonism to homocysteine in endothelial cell injury.The LDH of the middle and the low allicin groups was significantly lower than that of the homocysteine group(P<0.05).The antagonism of the high dose allicin group to homocysteine in endothelial cell injury was decreased at 36 hours.There was no significant difference between the high dose allicin group and the homocysteine group(P>0.05).The result of LDH coincided with that of MTT colorimetric method.Absorptiometry of the middle and the low allicin groups was significantly higher than that of the homocysteine group(P<0.01).There was no significant difference between the high dose allicin group and the homocysteine group(P>0.05).Conclusion: The level of homocysteine induced injury in endothelial cells is significantly decreased by low and middle doses of allicin,suggesting allicin may protect and cure homocysteinemia.