首页 | 本学科首页   官方微博 | 高级检索  
检索        

MSCs联合雷帕霉素对异基因鼠脾淋巴细胞的免疫调节作用
引用本文:殷玉俊,李晶,汤郁,尤海燕,朱伟,许文荣,焦志军.MSCs联合雷帕霉素对异基因鼠脾淋巴细胞的免疫调节作用[J].山东大学学报(医学版),2009,47(4):58-61.
作者姓名:殷玉俊  李晶  汤郁  尤海燕  朱伟  许文荣  焦志军
作者单位:殷玉俊,YIN Yu-jun(江苏大学附属医院皮肤科,江苏,镇江,212001);李晶,汤郁,LI Jing,TANG Yu(江苏大学附属医院风湿科,江苏,镇江,212001);尤海燕,焦志军,YOU Hai-yan,JIAO Zhi-jun(江苏大学附属医院检验科,江苏,镇江,212001);朱伟,许文荣,ZHU Wei,XU Weng-rong(江苏大学医学技术学院,江苏,镇江,212013)  
基金项目:江苏省科技厅社会发展项目 
摘    要:目的探讨体外骨髓间充质干细胞(MSCs)联合雷帕霉素对BALB/c鼠T、B淋巴细胞的免疫调节作用及可能机制。方法从5~6周龄BALB/c鼠骨髓中分离培养MSCs并鉴定其纯度。用EZ SepTM Mouse 1X分离异体BALB/c鼠脾脏淋巴细胞,分别在刀豆蛋白A(ConA)、脂多糖(LPS)刺激下,用MSCs和/或雷帕霉素处理,MTT法检测淋巴细胞的增殖,流式细胞术检测T、B淋巴细胞CD69、CD28和CD86的表达和T淋巴细胞凋亡情况,Real time PCR测定T淋巴细胞IL 10 mRNA、IFN γ mRNA的表达水平。结果MSCs明显抑制T、B淋巴细胞增殖,联合雷帕霉素组抑制作用更加显著(P<0.01)。MSCs可抑制T淋巴细胞的凋亡,联合雷帕霉素组较单独MSCs组抑制作用更加明显(P<0.01)。MSCs联合雷帕霉素具有协同促进IFN γ mRNA表达和协同抑制IL 10 mRNA表达的作用。单独MSCs或MSCs联合雷帕霉素对T、B淋巴细胞CD69、CD28和CD86的表达无显著影响。结论MSCs联合雷帕霉素对BALB/c鼠T、B淋巴细胞有免疫负调节作用,可能与协同抑制淋巴细胞增殖、T淋巴细胞凋亡和干预IFN γ mRNA、IL 10 mRNA表达有关。

关 键 词:间充质干细胞  雷帕霉素  淋巴细胞  免疫调节

Mesenchymal stem cells combined with rapamycin immuno-regulates the spleniclymphocytes in allogenic mice
YIN Yu-jun,LI Jing,TANG Yu,YOU Hai-yan,ZHU Wei,XU Weng-rong,JIAO Zhi-jun.Mesenchymal stem cells combined with rapamycin immuno-regulates the spleniclymphocytes in allogenic mice[J].Journal of Shandong University:Health Sciences,2009,47(4):58-61.
Authors:YIN Yu-jun  LI Jing  TANG Yu  YOU Hai-yan  ZHU Wei  XU Weng-rong  JIAO Zhi-jun
Institution:1. Department of Dermatology;2. Department of Rheumatology;3. Department Laboratory;Affiliated Hospital of Jiangsu University;Zhenjiang 212001;Jiangsu;China;4. School of Medical Technology;Jiangsu University;Zhenjiang 212013;China
Abstract:Objective To investigate the immunoregulatory effects of mesenchymal stem cells(MSCs) combined with rapamycin on T and B cells in vitro and its potential mechanism. Methods MSCs were isolated and cultivated from the bone marrow of 5-6 week-old BALB/c mice in vitro. The purity was detected through surface markers by flow cytometry(FCM). Splenic lymphocytes were isolated by EZ-Sep(tm) Mouse 1X. Under ConA or LPS stimulation,lymphocytes were treated with MSCs and/or rapamycin. The proliferation was assessed by...
Keywords:Mesenchymal stem cells  Rapamycin  Lymphocytes  Immunoregulatory  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《山东大学学报(医学版)》浏览原始摘要信息
点击此处可从《山东大学学报(医学版)》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号