嗅粘膜源性嗅鞘细胞移植联合NGF对脊髓损伤的修复

目的：探讨大鼠嗅粘膜嗅鞘细胞（olfactory ensheathing cells， OECs）移植联合神经生长因子(nerve growth factor， NGF)修复脊髓急性损伤的可行性和疗效，并观察二者的协同作用。方法：自成年Wistar大鼠嗅粘膜取材，采用差速贴壁和阿糖胞苷抑制纯化相结合的方法培养纯化嗅鞘细胞。建立大鼠脊髓半切模型，并随机分成对照组（A组），OECs组（B组）和OECs＋NGF组（C组），在不同时段进行肢体活动的BBB评分。8周后取脊髓标本进行组织学检查，评价对脊髓损伤的修复作用。结果：B组和C组2周后BBB评分高于A组（P＜0.05），C组自4周后明显优于A组和B组（P＜0.01）。组织学检查表明，C组可明显促进轴突再生。结论:OECs联合NGF具有良好的脊髓损伤修复作用，且二者具有明显的协同作用。

Repairs to the spinal cord by transplantation of olfactory ensheathing cells from the olfactory mucosa and NGF in adult rats

Objective: To investigate the effect of transplantation of olfactory ensheathing cells(OECs) originating from the olfactory mucosa and nerve growth factor(NGF) to repair spinal cords.Methods: The olfactory mucosa was separated from the nasal septum of adult Wistar rats.The OECs were purified by different attachment time with other types of cells and Ara-c inhibition,and the nerve growth factor receptor P75(P75NGFR) immunohistochemistry.The spinal cord samples were hemisected and they were randomly divided into three groups: the control group(group A),the olfactory ensheathing cell group(group B),and the olfactory ensheathing cells and the nerve growth factor group(group C).The Basso,Beattie,and Bresnahan locomotor rating scale(BBB) was observed at 2,4,6 and 8 weeks.Histological changes,the axon regeneration and the survival of OECs were determined 8 weeks later by an electron microscope,hematoxylin and eosin(HE) and immunohistochemistry staining.Result: The BBB grade of groups B and C was significantly higher than that of group A at 2 weeks,and that of group C was significantly higher than that of groups A and B.Histology revealed that the regeneration of axons was significantly promoted in group C.Conclusion: Transplantation of OECs and NGF is great for spinal cord repairs,and the two have a significant synergistic effect.