间充质干细胞在人胎盘组织中的染色定位

目的探索间充质干细胞(MSCs)在人胎盘组织中的分布规律。方法取正常剖宫产之足月健康产妇的新鲜胎盘组织,按中央带、中间带、边缘带、绒毛板层、中间层和基底板层分为9组:Aa、Ab、Ac、Ba、Bb、Bc、Ca、Cb、Cc。连续切片,以抗CD166、抗CD44、抗CD29分别做免疫荧光和免疫组化染色。显微镜下观察阳性细胞分布区域,采用HPIAS-1000彩色病理图像分析系统进行图像分析。结果CD166、CD44、CD29阳性表达细胞的分布情况较一致,主要集中于血管内皮下及血管附近间质内。胎盘中央带中间层(Ab)阳性细胞较集中,阳性表达的强度较强,其OD值与其它组差异有统计学意义(P<0.05)。边缘带阳性细胞稀少,阳性表达的强度较弱。结论胎盘各部位均存在CD166、CD44、CD29阳性表达的细胞,但脐带附着处下方胎盘中间层相对其他部位可能为MSCs富集区域。

Location of mesenchymal stem cells in human placentas: an experimental study

Objective To investigate the distribution pattern of mesenchymal stem cells(MSCs) in human placentas by immunofluorescence staining and immunohistochemical staining.Methods Fresh normal term placental tissues were taken from cesarean section without medical indication,and based on the central zone(A),the intermediate zone(B),the marginal zone(C),the chorionic plate layer(a),the middle layer(b) and the basal layer(c),they were divided into 9 groups: the Aa,Ab,Ac,Ba,Bb,Bc,Ca,Cb and Cc groups.Then,serial section,immunofluorescence staining and immunohistochemical staining were used to study the expressions of CD166,D44 and CD29.The HPIAS-1000 colored image analysis system was used to analyze distribution of positive expression cells.Results CD166,CD44 and CD29 positive cells were mainly concentrated in the area under vascular endothelial cells and in the stroma surrounding the vessel.There were many strongly positive cells in the intermediate zone and the middle layer of the placenta(Ab) and few positive cells in the marginal zone(C).Conclusion CD166,CD44 and CD29 positive cells could be found in every position in human placentas,but were mainly distributed in the intermediate zone and the middle layer of the placenta(Ab).