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鞘内注射VEGF对大鼠脊髓损伤后神经纤维和神经元的保护作用
引用本文:刘晓阳,孙建民,崔新刚,蒋振松.鞘内注射VEGF对大鼠脊髓损伤后神经纤维和神经元的保护作用[J].山东大学学报(医学版),2011,49(4):38-42.
作者姓名:刘晓阳  孙建民  崔新刚  蒋振松
作者单位:山东大学附属省立医院脊柱外科,济南,250021
基金项目:山东省自然科学基金资助项目
摘    要:目的探讨脊髓损伤后鞘内重复注射血管内皮生长因子(VEGF)对神经元和轴突的保护作用。方法将45只实验动物随机分为3组,VEGF组脊髓损伤后每天2次鞘内注射25 ng VEGF,连用1周。每周进行后肢BBB评分,8周后评价脊髓损伤的修复情况和VEGF的神经保护作用。结果手术后VEGF组动物后肢运动功能的恢复优于模型组,在后4周更为明显。VEGF组脊髓腹侧的神经纤维和神经元形态较好,损伤较轻,每高倍视野中血管数目明显多于模型组(P<0.001),caspase-3阳性细胞数少于模型组(P<0.001)。超微结构观察可见,VEGF组的神经纤维和神经元受损较轻,次级溶酶体的数量也明显少于模型组。结论 VEGF对大鼠脊髓损伤引起的神经纤维和神经元的变性有保护作用并可恢复损伤神经的部分功能。

关 键 词:鞘内注射  血管内皮生长因子  脊髓损伤  caspase-3  超微结构

Protective effect of intrathecal administration of VEGF on nerve fibers and neurons after spinal cord injury in rats
LIU Xiao-yang,SUN Jian-min,CUI Xin-gang,JIANG Zhen-song.Protective effect of intrathecal administration of VEGF on nerve fibers and neurons after spinal cord injury in rats[J].Journal of Shandong University:Health Sciences,2011,49(4):38-42.
Authors:LIU Xiao-yang  SUN Jian-min  CUI Xin-gang  JIANG Zhen-song
Institution:LIU Xiao-yang,SUN Jian-min,CUI Xin-gang,JIANG Zhen-song(Department of Spinal Surgery,Provincial Hospital Affiliated to Shandong University,Jinan 250021,China)
Abstract:Objective To investigate the effect of intrathecal administration of vascular endothelial growth factor(VEGF) on repair of spinal cord injury(SCI),and explore neuroprotection of VEGF on neurons and nerve fibers.Methods 45 animals were randomly divided into three groups.VEGF was intrathecally administrated at a dose of 25 ng twice a day for a week in the VEGF group after SCI.The Basso,Beattie and Bresnahan locomotor rating scale(BBB) was observed each week.8 weeks later,histological changes were examined by ...
Keywords:caspase-3
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