前炎性细胞因子基因多态性与乙型肝炎病毒感染的关系

目的探讨肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）基因单核苷酸多态性（SNP）与乙型肝炎病毒(HBV)感染的关系。方法采用基因芯片技术检测150例HBV感染患者（病例组）和不相关联的100例急性乙型肝炎病毒感染自行恢复后对照人群（对照组）中TNF-α-238G/A，-308G/A和IL-6-597G/A，-174G/C，-572G/C位点SNP。结果TNF-α-308GG基因型和G等位基因频率，病例组明显高于对照组（P＜0.01，相对危险度［OR］=6.71及P＜0.01，OR=2.22）；TNF 308AA基因型与抗病毒治疗具有协同作用（P＜0.05，OR=2.91）。未见IL -6和TNF-α其他位点的SNP与病例组有任何相关性。结论TNF-308GG基因型及其等位基因G与乙型肝炎病毒感染易感性相关，而TNF-308AA基因型则与抗病毒治疗具有协同抗病毒作用。

Relationship between proinflammatory cytokine gene polymorphisms and diseases of HBV infection

ObjectiveTo investigate relationships between the single nucleotide polymorphisms(SNPs) of tumor necrosis factor-α(TNFα) or interleukin-6(IL-6)promoter genes and susceptibility to patients with chronic hepatitis B (HB)in Jinan area. MethodsThe SNPs of the TNF-α(-238G/A and -308G/A) and IL-6(-597G/A, -174G/C and -572G/C) were determined by gene chips in 150 patients infected with hepatitis B virus(HBV) and 100 unrelated healthy controls of self limited HBV infection. ResultFrequencies of TNF-α-308GG genotype and G allele in patients with HBV infection were significantly higher than those in healthy controls（P＜0.01，OR=6.71， and P＜0.01，OR=2.22， respectively）. TNF-308AA genotype had a cooperative effect on the antiviral therapy （P＜0.05，OR=2.91）. No association was found between any of the other polymorphisms and patients with HBV infection. Conclusion TNF-α-308GG genotype and G allele are significantly related to patients with HBV infection, and TNF-308AA genotype is associated with the response to antiviral therapy.