| AMD3100对裸鼠结直肠癌肝转移模型中ICAM-1表达的影响 |
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| 引用本文： | 杨春康,黄小荣,简陈兴.AMD3100对裸鼠结直肠癌肝转移模型中ICAM-1表达的影响[J].黑龙江医学,2009,33(1). |
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| 作者姓名： | 杨春康 黄小荣 简陈兴 |
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| 作者单位： | 1. 福建医科大学教学医院、福建省肿瘤医院腹部外科,福建,福州,350011
2. 福建省莆田学院附属医院肿瘤外科,福建,莆田,351100 |
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| 摘 要： | 目的探讨CXCR4特异性抑制剂AMD3100对裸鼠结直肠癌肝转移模型中细胞间黏附分子-1(ICAM-1)表达的影响。方法取对数生长期的Lovo细胞接种于BALB/C裸鼠脾脏,建立结直肠癌肝转移模型。将18只模型裸鼠随机分入3组:对照组、低剂量治疗组、高剂量治疗组。对照组每天给予0.1 mL生理盐水腹腔注射,治疗组予0.1 mL AMD3100腹腔注射,低剂量治疗组0.1 mmol/L、高剂量治疗组0.15 mmol/L,1次/d,持续6周。处死实验鼠,制作肝脏转移瘤病理切片,用免疫组织化学方法检测各组I-CAM-1蛋白的表达情况。结果免疫组织化学显示,治疗组裸鼠结直肠癌肝转移模型中,ICAM-1蛋白表达较对照组降低。结论AMD3100可致裸鼠结直肠癌肝转移模型中ICAM-1蛋白的表达水平降低。
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| 关 键 词： | AMD3100 ICAM-1 免疫组织化学 |
Expressions of ICAM-1 in Nude Mice Model of Colorectal Cancer Liver Metastases Treated by AMD3100 |
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| YANG Chun-kang,HUANG Xiao-rong,JIAN Cheng-xing.Expressions of ICAM-1 in Nude Mice Model of Colorectal Cancer Liver Metastases Treated by AMD3100[J].Heilongjiang Medical Journal,2009,33(1). |
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| Authors: | YANG Chun-kang HUANG Xiao-rong JIAN Cheng-xing |
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| Institution: | YANG Chun-kang; HUANG Xiao-rong; JIAN Cheng-xing (Department of Abdominal Surgery; Fujian Provincial Tumor Hospital; Teaching Hospital of Fujian Medical University; Fuzhou 350011; China); |
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| Abstract: | Objective To investigate the expressions of ICAM-1(Intercellular Adhension Molecule-1) in nude mice model of colorectal cancer liver metastases treated by AMD3100.Methods Culture colon carcinomas cell line Lovo,and 18 nude mice model of colorectal cancer liver metastasis was established by splenectomy technique.Then mice were randomly divided into 3 groups: Control group(saline solution)、treatment group of low concentration AMD3100(0.1 mmol/L,0.1 mL/d) and treatment group of high concentration AMD3100(0.15 ... |
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| Keywords: | AMD 3100 ICAM-1 |
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