类风湿性关节炎灭活滑膜液细胞对非灭活滑膜液细胞γ-干扰素影响的初步研究

目的观察类风湿性关节炎(rheumatoid arthritis,RA)灭活滑膜液细胞对非灭活滑膜液细胞IFN-γ水平的影响。方法抽取RA患者膝关节液分离滑膜液细胞,分实验组:2.0×10~6/mL非灭活滑膜液细胞,分别与2.0×10~5/mL、4.0×10~4/mL、2.0×10~4/mL甲醛灭活后的滑膜液细胞(灭活滑膜液细胞比非灭活滑膜液细胞为1:10、1:50、1:100)混合培养;对照组:单独培养细胞数为2.0×10~6/mL的非灭活滑膜液细胞。37℃,5%CO_2条件下培养48 h后,ELISA法测定炎症因子IFN-γ水平。结果(1)实验组较对照组IFN-γ水平明显降低(P<0.05);(2)实验组各组间比较,灭活滑膜液细胞比非灭活滑膜液细胞为1:10组较1:50组及1:100组相比较IFN-γ水平显著降低(P<0.05)。1:50组同1:100组IFN-γ水平无显著性差异(P>0.05)。结论(1)灭活滑膜液细胞对非灭活滑膜液细胞IFN-γ的分泌具有抑制作用;(2)灭活滑膜液细胞比非灭活滑膜液细胞为1:10时,抑制IFN-γ分泌的作用最强;(3)灭活滑膜液细胞可抑制非灭活滑膜液的IFN-γ分泌,其机制有待进一步研究。

Effect of Inactivate Synovial Fluid Cell from Rheumatoid Arthritis Patients on IFN-γ Production of Activate Synovial Fluid Cell

Objective To observe the effect of inactivate synovial fluid cell from rheumatoid arthritis patients on IFN-γproduction of activate synovial fluid cell.Methods Synovial fluid in the knee joint of RA patients selected was drawn,then isolated the synovial fluid cells,which were randomly divided into treatment group and control group.In the treatment group,2.0×106/mL activate synovial fluid cells were mixed cultured separately with 2.0×105/mL、4.0×104/mL、2.0×104/mL inactivate synovial fluid cells,the ratio of inactivate synovial fluid cell to activate synovial fluid cell to be correspondingly 1：10、1：50、1：100.In the control group,2.0×106/mL activate synovial fluid cells were cultured purely.All above cells under the condition of 37℃and 5%CO_2 were cultivated for 48 hours.At last,the content of IFN-γwas determined by ELISA.Results（1） The IFN-γcontent was decreased obviously in the treatment groups compared with control groups（P〈0.05）.（2） The IFN-γlevel was significantly lower in the 1：10 group than that in the 1：50 and 1：100 group.There was no obvious difference about IFN-γbetween 1：50 and 1：100 group.Conclusion There might be a depressant effect of inactivate synovial fluid cell on IFN-γexcreted by synovial fluid cell in the rheumatoid arthritis patients.The depressant effect should be the strongest,when the ratio of inactive synovial fluid cell to active synovial fluid cell is 1：10.The mechanism shoul need to be investigated still further.