白细胞介素-6、10、17A在哮喘大鼠中的变化

目的建立大鼠哮喘模型,观察血清及支气管肺泡灌洗液IL-6、10、17A的水平变化,探讨其可能的作用机制。方法选用50只SD大鼠,随机分为正常对照组、哮喘模型组。模型组应用卵白蛋白（OVA）注射,雾化吸入致敏激发法复制哮喘模型。正常对照组取上清液备用。行支气管肺泡灌洗,回收支气管肺泡灌洗液（BALF）,吸取上清液备用。采用酶联免疫分析法测定血清及BALF上清液中IL-6、10、17A水平变化。结果两组大鼠血清细胞因子水平与肺泡灌洗液细胞因子水平,呈现出相同的统计学结果 ：哮喘模型组白细胞介素6、17A明显高于正常对照组（P〈0.05）,IL-10明显低于正常对照组（P〈0.01）。结论支气管哮喘的慢性气道炎症涉及多种细胞的相互作用,Th17细胞和其产生的IL-17促进了哮喘的发病,抑制过多的Th17细胞的生成,可能成为哮喘治疗的重要手段。

Changes of interleukin 6、10、17A level in asthmatic Rats

【Objective】To investigate the change of interleukin6、10、17A based on rat model of asthma and explore its mechanism of action.【Methods】Totally 50 health SD rats rando mLy divided into 2 groups： normal group, asthmatic model group. There were 25 rats in every group, and the numbers of the male and female rats are equal.The asthmatic models were established by ovalbumin（OVA） in asthmatic model group. The same dose of normal saline used in normal group replaced injection and inbreathe. The blood samples from the abdominal aorta were obtained in every group after the last inbreathe and supernatant were stored. Two group were given bronchoalveolar lavage and the supernatant of bronchoalveolar lavage fluid（BALF） were stored. The levels of interleukin-6、10、17A of serum and BALF were detected for every group. Interleukin-6、10、17A detected by enzyme linked immunoassay（ELISA）.【Resluts】50 rats were involved in the analysis of results. The levels of cytokine in serum and BALF display a same result of statistics： Contents of IL-6、17A in asthmatic model group were higher than those in normal group（P0.05）; Contents of IL-10 in asthmatic model group were lower than that in normal group（P0.01）.【Conclusion】The chronic airway inflammation of Bronchial asthma involved variety of cells the interaction, Th17 cells and IL-17 promotes asthma, inhibit excessive generation of Th17 cells may be an important means of treatment of asthma.