新生大鼠心肌细胞分离与原代培养

目的 应用酶消化法消化心肌组织、培养心肌细胞.方法 0.1%的胰蛋白酶重复消化乳鼠心肌组织多次,收集的细胞用含15%胎牛血清的MEM培养基混悬后过滤,纯化心肌细胞后置CO2培养箱孵育.结果 培养的心肌细胞4～6 h后开始贴壁生长,12～24 h明显增殖,3～4 d后细胞汇合成片.在倒置显微镜下心肌细胞呈圆形、梭形、多角形,伸出伪足,出现自发性节律性搏动.结论 应用酶消化法可简便、快速地获得大量活力高的心肌细胞.

Separation and Primary Culture of Neonate Rattus Cardiocyte

Objective To introduce how to culture cardiocytes after enzyme digestion of cardiac muscle. Methods Neonate rattus cardiac muscle was digested repeatedly by 0.1% trypsin. The cardiocytes were collected in MEM medium which contains 15% fetal bovine serum ,then suspended and filtered. The cardiocytes were placed into COz incubator to incubate after purification of them. Results Cardiocytes began to adhere and grow after 4-6 hours,to proliferate obviously after 12-24 hours, and to converge together after 3-4 days. The cardiocytes showed sphericalm, fusiform and polygon shape in the visual field of inverted microscope. All the cells stretched out parapodium and beated spontaneously and rhythmically. Conclusion A great quantity of vigorous cardiocytes can be cultured conviently and quickly by the application of enzyme digestion.