| 双自杀基因CD+TK对胰腺癌BxPC-3细胞的影响 |
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| 引用本文： | 张弘英,龚辉,谌珊珊,朱水山,李军.双自杀基因CD+TK对胰腺癌BxPC-3细胞的影响[J].江西医学院学报,2008,48(6). |
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| 作者姓名： | 张弘英 龚辉 谌珊珊 朱水山 李军 |
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| 作者单位： | [1]南昌大学第二附属医院消化内科,南昌330006 [2]南昌大学研究生院医学部2006级,南昌330006 |
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| 基金项目： | 江西省卫生厅资助课题
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| 摘 要： | 目的探讨逆转录病毒介导的双自杀基因对胰腺癌BxPC-3细胞的杀伤作用及胰腺癌的基因治疗方法。方法通过脂质体将含有双自杀基因的逆转录病毒载体MMLV—CD＋TK导入包装细胞PA317，经G418筛选后大量培养产病毒的阳性克隆PA317／CD＋TK细胞株，收集病毒上清液，浓缩后转染胰腺癌BxPG3细胞系，再次经G418筛选，获得稳定表达双自杀基因的BxPC-3／CD＋TK细胞株。用RT—PCR检测双自杀基因的表达。给予前体药物5-氟胞嘧啶（5-flourocytosine，5-FC）和（或）无环鸟苷（Ganciciovir，GCV）后，MTT法测定转基因组及未转基因组胰腺癌BxPC-3系细胞的存活率。结果双自杀基因在胰腺癌BxPC-3系细胞中可稳定表达，联合使用5-FC和GCV对细胞增殖的杀伤作用及旁杀伤效应效果明显。结论逆转录病毒介导自杀基因可有效杀死胰腺癌Bx-PC-3系细胞，双自杀基因具有更强的抗肿瘤作用。
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| 关 键 词： | 逆转录病毒 胰腺癌 BxPC-3细胞 基因治疗 |
Killing Effect of Cytosine Deaminase and HSV-TK Double Suicide Genes on Pancreatic Carcinoma Cells Mediated by Retrovirus Vector |
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| ZHANG Hong-yinga,GONG Huib,CHEN Shan-shana,ZHU Shui-shana,LI Juna.Killing Effect of Cytosine Deaminase and HSV-TK Double Suicide Genes on Pancreatic Carcinoma Cells Mediated by Retrovirus Vector[J].Acta Academiae Medicinae Jiangxi,2008,48(6). |
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| Authors: | ZHANG Hong-yinga GONG Huib CHEN Shan-shana ZHU Shui-shana LI Juna |
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| Institution: | a.Department of Gastroenterology;the Second Affiliated Hospital;b.2006 Grade of Medical Department of Graduate School;Nanchang University;Nanchang 330006;China |
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| Abstract: | Objective To investigate the different killing effects on human pancreatic carcinoma BxPC-3 cells with cytosine deaminase(CD)and herpes simplex virus-thymidine kinase(HSV-TK)double suicide genes co-expression compared with single gene mediated by retrovirus vector.To find a more efficient and low-cytotoxicity suicide gene therapy for pancreatic carcinoma.MethodsCD and HSV-TK double suicide genes were transfected into BxPC-3 cells using lipofectamine.The positive clones were picked out and cultured after G41... |
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| Keywords: | retroviridae pancreatic carcinoma BxPC-3 cells gene therapy |
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