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改良荧光原位杂交技术检测乳腺癌HER2基因
引用本文:彭大云,张伟,陈晓东,赖日权,肖辉.改良荧光原位杂交技术检测乳腺癌HER2基因[J].华南国防医学杂志,2010,24(3):208-210.
作者姓名:彭大云  张伟  陈晓东  赖日权  肖辉
作者单位:广州军区广州总医院病理科,广东广州,510010
摘    要:目的探讨改良荧光原位杂交(fluorescent in situ hybridization,FISH)技术在检测乳腺癌HER2基因中的应用。方法对52例经免疫组化(immunohistochemistry,IHC)法检测HER2的乳腺癌标本行改良FISH法检测HER2基因扩增情况,Kappa检验法对两种检测结果的一致性进行统计分析。结果改良FISH法成功率高,与IHC法检测结果一致性好,两者实际一致率为88.5%(K=0.757,P=0.000)。结论改良FISH技术可作为最终确诊HER2基因状态的方法 。

关 键 词:荧光原位杂交  免疫组化  乳腺癌  HER2基因

Improved Fluorescent in situ Hybridization in Detection of Breast Cancer HER2 Gene
PENG Da-yun,ZHANG Wei,CHEN Xiao-dong,LAI Ri-quan,XIAO Hui.Improved Fluorescent in situ Hybridization in Detection of Breast Cancer HER2 Gene[J].Military Medical Journal of South China,2010,24(3):208-210.
Authors:PENG Da-yun  ZHANG Wei  CHEN Xiao-dong  LAI Ri-quan  XIAO Hui
Institution:.Department of Pathology,Guangzhou General Hospital of Guangzhou Military Command,Guangzhou Guangdong 510010,China
Abstract:Objective To investigate the implication of improved fluorescent in situ hybridization (FISH) technique in the detection of HER2 gene in breast cancer tissues.Methods HER2 genes amplification in 52 samples with breast cancer detected by IHC method was tested by improved FISH method.Kappa test was employed to compare the results of the two methods.Results Improved FISH method had high success rate and high coincidence with IHC method.The concordance rate was 88.5% (K=0.757,P=0.000).Conclusion The improved FISH is simple to operate,safe,stable,and highly sensitive in detecting HER2 gene amplification of breast cancer tissue.It can be utilized to diagnose HER2 gene status.
Keywords:Fluorescence in situ hybridization  Immunohistochemistry  Breast cancer  HER2 gene
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