| 实时定量PCR检测口腔组织中CD44 mRNA的表达 |
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| 引用本文： | 张莉芹,田彩平,马世红,袁红霞.实时定量PCR检测口腔组织中CD44 mRNA的表达[J].甘肃医药,2014,33(1):9-11. |
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| 作者姓名： | 张莉芹 田彩平 马世红 袁红霞 |
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| 作者单位： | 张莉芹 (甘肃省肿瘤医院门诊口腔科,甘肃兰州,730050)； 田彩平 (甘肃省医学科学研究院医学分子生物学研究中心,甘肃兰州,730050)； 马世红 (甘肃省肿瘤医院头颈外科,甘肃兰州,730050)； 袁红霞 (甘肃省医学科学研究院医学分子生物学研究中心,甘肃兰州,730050)； |
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| 基金项目： | 甘肃省科技支撑计划资助项目(项目编号：1104FKCA134) |
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| 摘 要： | 目的：研究细胞黏附分子CD44mRNA在口腔组织中的表达情况,探讨CD44与口腔鳞癌之间的关系.方法：应用实时定量PCR（real-time PCR,RT-PCR）检测17例正常口腔组织及14例口腔鳞癌组织中CD44mRNA的表达情况.结果：CD44 mRNA在口腔鳞癌组织中的表达显著低于正常口腔组织中的表达（P=0.038）;与正常口腔组织中CD44mRNA的表达水平相比较,其中有12例（86％）口腔鳞癌组织表达下调,2例（14％）口腔鳞癌组织表达上调.结论：口腔鳞癌组织中CD44mRNA的表达显著低于正常口腔组织中的表达,因此CD44的表达量可能与口腔鳞癌的发病及转移有关.
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| 关 键 词： | 口腔组织 CD44 mRNA 实时定量PCR |
Detection the expression of CD44 mRNA in Oral Tissues by Real-Time PCR |
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| Authors: | ZHANG Li-qin TIAN Cai-ping MA Shi-hong YUAN Hongxia |
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| Institution: | 1. Outpatient Emergency Department ot Gansu Province Tumor Hospital;2. Medical Molecular Biology Research Center of Gansu Province Medical Science Institute;3. Head and Neck Surger Department of Gansu Province Tumor Hospital,Lanzhou 730050, China) |
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| Abstract: | Objective：In order to study the relationship between CD44 and oral squamous cell carcinomas, in this paper we discuss the expression of CD44 mRNA in oral tissues. Methods： Detected the expression level of CD44 mRNA in 17 cases of normal oral tissues and 14 cases of oral squamous carcinoma tissue by real-time PCR. Results： The expression level of CD44 mRNA in oral squamous carcinoma tissues was significantly lower than in normal oral tissues （P=0.038）;Compared with the expression level of CD44 mRNA in nomlal oral tissues,There had 12 cases （86%） decreased but had 2 cases increased in oral squamous carcinoma tissues. Conclusion： The expression level of CD44 mRNA in oral squamous carcinoma tissues was significantly lower than in normal oral tissues, So the expression level of CD44 may be associated with the morbidity and metastasis of oral squamous cell carcinomas. |
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| Keywords: | Oral tissues CD44 mRNA Real-time PCR |
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